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EC number: 248-698-8
CAS number: 27859-58-1
Table 2: Cell Growth data
Nominal Concentration of Test Material(mg/L)
Number of Cells per Millilitre(Mean of replicate flasks)
Table 3: Average Specific Growth Rate
Average Specific Growth Rate
Percent of Control
The acute toxicity of the water accommodated fraction (WAF) of the test
material to the freshwater alga, Selenastrum capricornutum, was
investigated during a range-finding study; performed under GLP
conditions and to the standardised guideline OECD 201.
The test, which was designed to establish the approximate toxicity of
the test substance, was performed for 96 hours at 24 ± 1 °C under static
conditions with a control (0 mg/L) and five concentrations of WAF (0.3,
3.0, 33, 330, and 3,300 mg/L).
The dilution water was sterile enriched media adjusted to a pH of 7.5 ±
0.1. The five WAFs were prepared by formulating individual
concentrations of the test substance and dilution water in mixing
vessels equipped with a magnetic stirrer, stirring the mixtures for
approximately 20 hours, settling the mixtures for approximately four
hours, and siphoning the water phase containing the WAF. During the
mixing period, the vortex extended approximately 25 % of the distance
from the water surface to the bottom of the mixing vessels.
Insoluble material was observed at 24, 72, and 96 hours in vessels
containing 330 mg/L and 3,300 mg/L test media (test solutions were
turbid, and turbidity increased with concentration). No other insoluble
material was noted during the test.
Exposure of freshwater alga to the test material resulted in a 96 hour
median effective concentration (EC50) of 93 mg/L (95 % confidence
interval = 33 to 330 mg/L) when calculated using cells/mL, and 100 mg/L
(95 % confidence interval = 33 to 330 mg/L) when calculated using
average specific growth rate.
Under the conditions of this study, the 96 hour no observed effect
concentration (NOEC) was determined to be 33 mg/L using both cells/mL
and average specific growth rate.
The 96 h EC50 was 100 mg/L based on growth rate. The 96 h NOEC was determined to be 33 mg/L.
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