Registration Dossier

Environmental fate & pathways

Endpoint summary

Administrative data

Description of key information

The test material has been determined not to be readily biodegradable.

Additional information

As part of an investigation to determine the ready biodegradability of a number of substances, the ready biodegradability of the test material was investigated in a respirometry test that followed the same principle as that in the standardised guideline OECD 301 F, except that it did not include the abiotic and toxicity checks and pre-adapted inoculum was employed. The study was conducted under GLP conditions.

The inoculum was adapted to the test material for 14 days before test initiation and the degradability was expressed as the ratio of the measured Biological Oxygen Demand (BOD) and the Theoretical Oxygen Demand (ThOD; calculated from the elemental analysis of the substance).

The initial test material concentration was 100 mg/L and the temperature throughout the 28 day test was 23 ± 1 °C. Oxygen consumption was measured with a BI-1000 electrolytic respirometer system; the oxygen uptake in all flasks were monitored continuously and recorded automatically every 4 hours throughout the test period. Sodium benzoate was employed as reference material.

The level of biodegradation at the end of the 28 day test period for the test material was 18.3 % (average of two replicates). The reference substance reached a biodegradation level of 94.3 % in the same test period.

Under the conditions of the study, the test material has been determined not to be readily biodegradable.