(2E)-2-ethyl-4-(2,2,3-trimethylcyclopent-3-en-1-yl)but-2-en-1-ol

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

fish early-life stage toxicity

Type of information:

(Q)SAR

Adequacy of study:

key study

Study period:

7 October 2015 to 15 October 2015

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

accepted calculation method

Remarks:

Value obtained by QSAR method.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)

Principles of method if other than guideline:

The chronic toxicity to fish was determined using a validated QSAR for the Mode of Action in question, (MOA 1, non-polar narcosis). Use of this MOA for unsaturated alcohols has previously been demonstrated as acceptable in a position paper (Bauer et al., 2015) (2). The QSAR is based on validated data for a training set of 26 chemicals derived from 14 to 32-day test on fish, for which the concentrations of the test item had been determined by chemical analyses over the test period.

GLP compliance:

no

Remarks:

Not applicable

Analytical monitoring:

no

Details on sampling:

Not applicable

Vehicle:

no

Details on test solutions:

Not applicable

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Also applicale: Pimephales promelas, Oryzias latipes, Leuciscus idus, Jordanella floridae, Cyprinus carpio

Test type:

other: Flow-through, semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

28 d

Remarks on exposure duration:

median duration: 14 to 32 days

Post exposure observation period:

Not applicable.

Hardness:

The QSAR is based on data from studies performed at acceptable hardness to ensure control survival.

Test temperature:

The QSAR is based on data from studies performed between 21 and 27°C depending on species used.

pH:

The QSAR is based on data from studies performed between 6.0 and 8.5.

Dissolved oxygen:

The QSAR is based on data from studies performed at acceptable oxygen concentrations (generally >60%).

Salinity:

Not applicable

Conductivity:

Not applicable

Nominal and measured concentrations:

The QSAR is based on data from studies performed using measured concentrations or with acceptable stability.

Details on test conditions:

Not applicable

Reference substance (positive control):

not required

Remarks:

QSAR

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

ca. 0.23 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks:

or growth

Remarks on result:

other: 95%CL: 0.17-0.31

Details on results:

Not applicable

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

95% confidence interval: 0.17 - 0.31 mg/L
Qsar statitical parameters are given in the QPRF

No data

Validity criteria fulfilled:

yes

Remarks:

The substance falls into applicability domains of the QSAR model.

Conclusions:

The 28d-NOEC value based on measured concentrations has been calculated as 0.23 mg/L.

95%CL: 0.17-0.31 mg/L

Executive summary:

A QSAR prediction was performed to assess the chronic toxicity of the test item to fish. This QSAR has been validated to be compliant with the OECD recommendations for QSAR modeling (OECD, 2004) and predicts the endpoint value which would be expected when testing the substance under experimental conditions in a laboratory following Guideline for Testing of Chemicals No. 210, "Fish, Early-life Stage Toxicity Test" (1). The criterion predicted was the NOEC (No Observed Effect Concentration), a tested concentration which is observed to cause no effect on growth or reproducibility within a period of 28 days. The chronic toxicity to fish of the test item has been investigated using a QSAR model that predicts fish lethality in an OECD 210 study. The test item falls within the applicability domain of the model as demonstrated in the QPRF. The 28d-NOEC = 0.23 mg test item/L with 95%-Confidence Limit of 0.17 -0.31 mg test item/L. This toxicity study is acceptable and can be used for that endpoint.

Results Synopsis

Test Type: QSAR model

NOEC: 0.23 mg test material/L

95%-C.I.: 0.17 to 0.31 mg test material/L

Description of key information

The 28d-NOEC has been calculated as 0.23 mg/L using a valid QSAR.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.23 mg/L

Additional information

One QSAR data is available to determine the long-term toxicity to fish.

A QSAR prediction was performed to assess the chronic toxicity of the test item 2-ethyl-4-(2,2,3-trimethyl-3-cyclopenten-1-yl)-(2E)-buten-1-ol to fish. This QSAR has been validated to be compliant with the OECD recommendations for QSAR modeling (OECD, 2004) and predicts the endpoint value which would be expected when testing the substance under experimental conditions in a laboratory following Guideline for Testing of Chemicals No. 210, "Fish, Early-life Stage Toxicity Test" (1). The criterion predicted was the NOEC (No Observed Effect Concentration), a tested concentration which is observed to cause no effect on growth or reproducibility within a period of 28 days. The chronic toxicity to fish of 2-ethyl-4-(2,2,3-trimethylcyclopent-3-en-1-yl)but-2-en-1-ol has been investigated using a QSAR model that predicts fish lethality in an OECD 210 study. 2-ethyl-4-(2,2,3-trimethylcyclopent-3-en-1-yl)but-2-en-1-ol, falls within the applicability domain of the model as demonstrated in the QPRF. The 28d-NOEC = 0.23 mg test item/L with 95%-Confidence Limit of 0.17 -0.31 mg test item/L. This toxicity study is acceptable and can be used for that endpoint.