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Diss Factsheets
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EC number: 224-638-6 | CAS number: 4433-79-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed OECD and GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4'-chloro-2',5'-dimethoxyacetoacetanilide
- EC Number:
- 224-638-6
- EC Name:
- 4'-chloro-2',5'-dimethoxyacetoacetanilide
- Cas Number:
- 4433-79-8
- Molecular formula:
- C12H14ClNO4
- IUPAC Name:
- N-(4-chloro-2,5-dimethoxyphenyl)-3-oxobutanamide
- Details on test material:
- - Name of test material (as cited in study report): Naphthol AS-IRG
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- Origin: Chemisch pharmazeutische Fabrik Dr. K. Thomae, 7950 Biberach
Animal identification: numbered ear tags
Weights: 2.7 - 3.2 kg
Age: about 3 - 5 months
Housing: in fully air-conditioned rooms, separate cages (arranged in a battery)
Temperature: 18 +/- 3 °C
Relative humidity: 55 +/- 20 %
Lighting time: 12 hrs per day
Diet: Altromin 2123 maintenance diet - rabbits (Altromin GmbH, Lage/Lippe), ad libitum
Water: deionized, chlorinated water from automatic water dispensers, ad libitum
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- shaved
- Vehicle:
- other: polyethylene glycol 400
- Controls:
- not required
- Amount / concentration applied:
- 0.5 g Naphtol pasted with 0.3 ml polyethylene glycol 400
- Duration of treatment / exposure:
- Exposure period: 4 hrs. After the exposure period all remnants of the test substance were carefully removed from the skin with warm tap water.
- Observation period:
- Total observation period: 72 hrs.
Observations at: 30-60 min, 24, 48 and 72 hrs after removal of patches - Number of animals:
- 3
- Details on study design:
- Type of wrap: cellulose patch
Area exposed: 2.5 x 2.5 cm
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- other: mean of animal # 72, 176, 177 each
- Time point:
- other: Mean of 24, 48, 72 h
- Score:
- 0
- Reversibility:
- other: initial slight effects were fully reversible wihin 24 h
- Irritation parameter:
- edema score
- Basis:
- other: mean of animal # 72, 176, 177 each
- Time point:
- other: Mean of 24, 48, 72 h
- Score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- other: no effects at any time
- Irritant / corrosive response data:
- No signs of irritation/corrosion except very slight erythema (at 1h) have been observed during the entire study period.
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- Not irritating
- Executive summary:
The test item was tested for skin irritating or skin corrosive properties according to OECD 404. Following this protocol 500 mg Naphthol AS-IRG mixed with 0.3 ml polyethylene glycol 400 were applied to the shaved skin of New Zealand White rabbits. After 4 h exposure time the semiocclusive covering was removed and remaining substance was removed from the skin with warm tap water. Mean scores (mean of 24, 48, 72 h) were claculated for oedema and erythema for each rabbit.
All calculated mean scores were 0. Based on these findings the test substance is neither skin irritating nor skin corrosing in this test.
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