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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: OECD guideline followed to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
test volume increased and closed test vessels used to minimise loss of test substance by volatilisation
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
yes
Remarks:
test volume increased and closed test vessels used to minimise loss of test substance by volatilisation
GLP compliance:
yes (incl. QA statement)
Remarks:
Copy of certificate included

Test material

Constituent 1
Chemical structure
Reference substance name:
Buta-1,2-diene
EC Number:
209-674-2
EC Name:
Buta-1,2-diene
Cas Number:
590-19-2
Molecular formula:
C4H6
IUPAC Name:
buta-1,2-diene
Details on test material:
97.91% purity, manufactured in 1998 with a stability of >2 years
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data reported

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Samples taken at start and end of exposure period

Test solutions

Vehicle:
no
Details on test solutions:
For the prepartation of a stock solution, appr. 2ml of the test substance were mixed with 200ml deionised water and occasionally stirred during 18h, all at 4°C. This solution was diluted with deionised water at a temperature of 20°C. The resulting solution served as stock solution with a measured analytical value of 66.7mg TOC/l resp. 75.0mg substance/l. 5 test concentrations and a control.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
clone 5, bred in house, test organisms <24 hours old. "0 organissma in 4 parallel sets of 5 (±1) each

Study design

Test type:
other: Closed, static
Water media type:
freshwater
Total exposure duration:
48 h
Post exposure observation period:
None

Test conditions

Hardness:
250 mg/l CaCO3
Test temperature:
20°C ±1°C
pH:
7.7-8.2
Dissolved oxygen:
86-101 %
Salinity:
N/A
Nominal and measured concentrations:
nominal concentrations 3.3, 5.0, 6.7, 10.0 and 13.3mg.l-1. Geometric mean measured concentrations 2.8, 3.3, 4.5, 5.6 and 7.8mg.l-1
Details on test conditions:
To minimise the loss of the volatile test substance during the test, the volume inside the test vessels was increased to 20ml resulting in a reduced gas space. The vessels were closed tightly (gas tight septum).
Reference substance (positive control):
yes
Remarks:
potassium dichromate is tested as a reference substance quarterly

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7.3 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 7.8 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Highest test concentration
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
5.6 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
As the nominal and measured concentrations deviated by more than 20% the effect concentration is reported as the geometric mean of the start and end concentrations.
Results with reference substance (positive control):
1mg K2Cr2O7 ≤50% effect.
2mg K2Cr2O7 ≥50% effect.
Reported statistics and error estimates:
No data reported

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Fulfills validity criteria
Conclusions:
The reported 48hr EC50 with buta-1,2-diene is 7.3mg/l
Executive summary:

The reported 48hr EC50 with buta-1,2-diene is 7.3mg/l. This study is GLP compliant and follows a standard guideline. In addition, the data required to assess validity has been included.