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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08 May - 23 Jun 1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Reference:
Composition 1
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
not specified
Test material information:
Composition 1
Specific details on test material used for the study:
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Solubility and stability of the test substance in the solvent/vehicle: soluble in alcohol and ether
Oxygen conditions:
aerobic
Inoculum or test system:
mixture of sewage, soil and natural water
Details on inoculum:
Preparation according to MITI standard procedure (Mixed inoculum from 10 sites: 4 sewage plants, 3 rivers, 1 lake and 2 bays around Japan)
Inoculum test concentration: 30 mg/L
Duration of test (contact time):
2 wk
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
TEST SYSTEM
- Culturing apparatus: closed oxgen consumption measuring machine with 300 mL culture bottle
- Measuring equipment: Total organic carbon analyzer

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
2 wk
Parameter:
% degradation (test mat. analysis)
Value:
2.1
Sampling time:
2 wk
Interpretation of results:
not readily biodegradable

Description of key information

not readily biodegradable: 2.1% after 28 d

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

There is one study available, which investigated the biodegradation of the test substance in water according to OECD 301C. Microorganisms were inoculated with 100 mg/L test substance in closed bottles for two weeks and oxygen consumption as well as GC/FID was followed to estimate biodegradation.

After two weeks the test substance was degraded by 2.1% based on analysis of the test substance and 0% based on O2 consumption. Therefore, it was concluded that the test substance is not readily biodegradable according to the OECD criteria.