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EC number: 211-661-1 | CAS number: 682-09-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 June to 19 July 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Proprietary GLP and guideline-compliant study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,2-bis(allyloxymethyl)butan-1-ol
- EC Number:
- 211-661-1
- EC Name:
- 2,2-bis(allyloxymethyl)butan-1-ol
- Cas Number:
- 682-09-7
- Molecular formula:
- C12H22O3
- IUPAC Name:
- 2,2-bis[(prop-2-en-1-yloxy)methyl]butan-1-ol
- Details on test material:
- TMPDE. The trade name of the substance was NEOALLYL T-20, lot no. 30341, received on 7 June 1993. The substance was a mixture of Trimethylolpropane Diallyl Ether (87.4%), Trimethylolpropane Monoallyl Ether (6.9%), and Trimethyolpropane Triallyl Ether (5.7%). The purity was given as 100%. The substance was described as a colourless liquid, and was stored in a metal canister at 4°C in the dark.
Constituent 1
Method
- Target gene:
- Histidine and tryptophan auxotrophic bacterial strains were used
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix obtained from the British Industrial Biological Research Association, prepared from the livers of Aroclor 1254 induced male Sprague-Dawley rats
- Test concentrations with justification for top dose:
- Preliminary study: 0, 312.5, 625, 1250, 2500 and 5000 µg/plate.
Mutation study experiment 1: 0, 8.0, 40, 200, 1000 and 5000 µg/plate.
Mutation study experiment 2: 0, 312.5, 625, 1250, 2500 and 5000 µg/plate. - Vehicle / solvent:
- The test substance was accurately weighed and dissolved in dimethyl sulphoxide and appropriate dilutions made. The test material preparations were prepared according to the proportion of the major component of the mixture, the dose levels therefore corresponded to the concentration of trimethylolpropane diallyl ether. Test concentrations were used within 90 minutes of preparation.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- Migrated to IUCLID6: without S9; WP2uvrA-, TA100 and TA1535
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Migrated to IUCLID6: without S9; TA1537
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Remarks:
- Migrated to IUCLID6: without S9; TA98
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene, with S9; Ta1535 ad WP2uvrA-
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- Migrated to IUCLID6: with S9; TA100, TA1537 and TA98
- Details on test system and experimental conditions:
- Overnight sub cultures of the appropriate stock cultures were prepared in nutrient broth (Oxoid Ltd.) and incuated at 37°C for 10 hours.
Top agar was prepared using 0.6% Difco Bacto agar and 0.5% sodiumn chloride. For the Salmonella strains, 5 ml of 1.0 mM histidine/1.0 mM biotin solution was added to each 100 ml of top agar, and for E. coli 5 ml of 1.0 mM tryptophan was added to each 100 ml of top agar. Base agar plates were prepared using 1.2% Oxoid Agar Technical No. 3 with Vogel-Bonner Medium E and 20 mg/ml D-glucose.
Preliminary study: a preliminary test was carried out to determine the toxicity of the test substance. 0.1 ml bacterial suspension (TA100 or WP2uvrA-), 0.1 ml test solution and 0.5 ml phosphate buffer were added to 2 ml molten, trace histidine/tryptophan supplemented media and overlayed onto sterile plates of Vogel-Bonner minimal agar ( 30 ml/plate). Five doses of the test material and a solvent control (DMSO) were tested in duplicate. After 48 hours incubation at 37°C the plates were scored for revertant colonies and examined for a thinning of the background lawn.
Mutation study Experiment 1: Five concentrations of the test substance were assayed in triplicate against each tester strain using the direct plate incorporation method. 0.1 ml aliquots of bacterial suspension were dispensed into sets of sterile test tubes containing 2.0 ml of molten trace histidine/tryptohphan supplemented top agar at 45°C. These sets comprised two test tubes for each bacterial tester strain. 0.1 ml test substance or negative control was also added followed by either 0.5 ml S9 mix or 0.5 ml of pH 7.4 buffer. The contents of each tube were mixed and equally distributed onto the surface of Vogel-Bonner agar plates. Positive controls were plated in an identical manner. The plates were then incubated at 37°C for 48 hours and the number of revertant colonies counted was counted using an automated colony counter.
Mutation study Experiment 2: The second experiment was performed according to the methods used in the first experiment, using fresh bacterial cultures, test material and control solutions (in triplicate). - Evaluation criteria:
- Toxicity was evaluated by a thinning of the background lawn.
Mutagenicity was evaluated by the number of revertant colonies. A substance was considered positive if it induced a dose-related doubling of the spontaneous reversion rate in one or more strains in both experiments. - Statistics:
- Statsitical evaluation was not required.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- Preliminary study: the mean numbers of revertant colonies for the toxicity assay were not affected by the test substance tested up to a concentration of 5000 µg/plate, therefore the test substance was considered to be non-toxic to strains TA100 and WP2uvrA-.
Main study: the results of the checks for characteristics, viability and spontaneous reversion rate for each tester strain were all found to be satisfactory. No toxicity was exhibited to any of the strains of bacteria used.
No significant increases in the number of revertant colonies of bacteria were recorded for any of the strains of bacteria used, at any dose level, either with or without metabolic activation. The positive control substances all produced marked increases in the number of revertant colonies and the activity of the S9 fraction was found to be satisfactory. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
No significant increases in the number of revertant colonies of bacteria were recorded for any of the strains of bacteria used, at any dose level, either with or without metabolic activation.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
The test substance was found to be non-mutagenic under the conditions of this test. - Executive summary:
The mutagenic potential of TMPDE (NEOALLYL T-20) was determined in the Ames test, according to OECD method 471. Salmonella typhimurium strains TA1535, TA1537, TA98 and TA100, and Escherichia coli strain WP2uvrA- were treated with the test substance by the plate incorporation method at five dose levels (up to a maximum concentration of 5000 µg/ml). Test concentrations were plated in triplicate for each strain, both with and without exogenous metabolic activation (S9 mix). The test substance was found to be non-toxic to TA100 and WP2uvrA, when tested up to a concentration of 5000 µg/ml in a preliminary experiment with and without metabolic activation. The mutation assay was repeated in an independent experiment using fresh bacterial cultures and test substance preparations. The solvent (dimethyl sulphoxide) control plates gave counts of revertant colonies within the normal range. All positive control chemicals produced marked increased in the numbers of revertant colonies, both with and without metabolic activation. No significant increase in the numbers of revertant colonies was recorded for any strain with any dose of test substance, either with or without metabolic activation. The test substance was found to be non-mutagenic under the conditions of this test.
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