Registration Dossier
Registration Dossier
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EC number: 210-088-4 | CAS number: 605-50-5
Toxicological information
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 June - 28 July 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Official notice of J MHLW, METI and ME (21 November 2003): YAKUSHOKUHATSU No.1121002, SEIKYOKU No.2, KANPOKIHATSU No. 031121002
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Diisoamyl phthalate
- IUPAC Name:
- Diisoamyl phthalate
- Details on test material:
- - Name of test material (as cited in study report): Diisoamyl phthalate
- Substance type: organic
- Physical state: liquid
- Analytical purity: 100%
- Lot/batch No.: 7920-1-1
- Expiration date of the lot/batch:12 August 2012
- Storage condition of test material: room temperature in the dark
Constituent 1
Method
- Target gene:
- Histidine operon (S. typhimurium)
Tryptophane operon (E. coli)
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- E. coli WP2 uvr A pKM 101
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver homogenate (S9 mix)
- Test concentrations with justification for top dose:
- 5, 15, 50, 150, 500, 1500, 5000 µg per plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Substance dissolved in DMSO but not in water.
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Absence of S9 mix
2 µg/plate for strains TA100 and TA1535
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Absence of S9 mix
50 µg/plate for strain TA1537
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- Absence of S9 mix
2 µg/plate for strains TA98
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Remarks:
- Absence of S9 mix
2 µg/plate for strain WP2 uvrA (pKM101)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene 5 µg/plate for strains TA100 and TA1535 and 10 µg/plate for strain WP2 uvrA (pKM101).
- Remarks:
- Presence of S9 mix.
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- Presence of S9 mix
5 µg/plate for strains TA98 and TA1537
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation) for test 1; preincubation for test 2
DURATION
- Preincubation period: 0 hours for the first test and 0.5 hours for the second test
- Exposure duration: 72 hours at 37°C
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth - Evaluation criteria:
- If exposure to a test substance produces a reproducible increase in revertant colony numbers of at least twice (three times in the case of strains TA1535 and TA1537) the concurrent vehicle controls, with some evidence of a positive dose-response relationship, it is considered to exhibit mutagenic activity in this test system.
- Statistics:
- No statistical analysis is performed.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
None noted
RANGE-FINDING/SCREENING STUDIES:
None performed
COMPARISON WITH HISTORICAL CONTROL DATA:
The mean revertant colony counts for the vehicle controls were within or close to the 99% confidence limits of the current historical control range of the laboratory. Appropriate positive control chemicals (with S9 mix where required) induced substantial increases in revertant colony numbers with all strains in all reported tests, confirming sensitivity of the cultures and activity of the S9 mix. See attachments in background material below for historic control data
RESULTS
First test: No evidence of toxicity was obtained following exposure to diisoamyl phthalate following exposure to diisoamyl phthalate. A maximum exposure concentration of 5000 µg/plate was, therefore, selected for use in the second test.
No substantial increases in revertant colony numbers over control counts were obtained with any of the tester strains following exposure to diisoamyl phthalate at any concentration up to and including 5000 µg/plate in either the presence or absence of S9 mix.
Second test: No evidence of toxicity was obtained following exposure to diisoamyl phthalate following exposure to diisoamyl phthalate.
No substantial increases in revertant colony numbers over control counts were obtained with any of the tester strains following exposure to diisoamyl phthalate at any concentration up to and including 5000 µg/plate in either the presence or absence of S9 mix.
Any other information on results incl. tables
| Results obtained in the absence of metabolic activation: test 1 | ||||||
| Study number: REI0008 | ||||||
| Experiment: Test 1 | ||||||
| Assay conditions: Plate incorporation assay | ||||||
| Without metabolic activation | ||||||
| Strain | Addition | Concentration per plate | Mean revertants per plate | Standard Deviation | Fold increase relative to vehicle | Individual revertant |
| colony counts | ||||||
| TA98 | DMSO | 33 | 4.6 | 32, 29, 38 | ||
| Diisoamylphthalate | 5 µg | 32.3 | 4.5 | 1 | 37, 28, 32 | |
| 15 µg | 30 | 4.4 | 0.9 | 35, 28, 27 | ||
| 50 µg | 35.3 | 3.5 | 1.1 | 39, 32, 35 | ||
| 150 µg | 35.3 | 4.5 | 1.1 | 40, 31, 35 | ||
| 500 µg | 30 | 2.6 | 0.9 | 29, 33, 28 | ||
| 1500 µg | 22.3 | 0.6 | 0.7 | 23, 22, 22 | ||
| 5000 µg | 28.7 | 2.1 | 0.9 | 28, 31, 27 | ||
| TA100 | DMSO | 152.3 | 16.5 | 169, 136, 152 | ||
| Diisoamylphthalate | 5 µg | 139.7 | 1.2 | 0.9 | 139, 139, 141 | |
| 15 µg | 133.7 | 5.8 | 0.9 | 127, 137, 137 | ||
| 50 µg | 128.3 | 21.2 | 0.8 | 143, 138, 104 | ||
| 150 µg | 149 | 6 | 1 | 155, 143, 149 | ||
| 500 µg | 138 | 18.2 | 0.9 | 128, 127, 159 | ||
| 1500 µg | 148.3 | 7 | 1 | 155, 141, 149 | ||
| 5000 µg | 135.7 | 7.8 | 0.9 | 138, 127, 142 | ||
| TA1535 | DMSO | 26.7 | 0.6 | 27, 26, 27 | ||
| Diisoamylphthalate | 5 µg | 18.7 | 5.1 | 0.7 | 20, 13, 23 | |
| 15 µg | 20.3 | 2.9 | 0.8 | 17, 22, 22 | ||
| 50 µg | 22.7 | 9 | 0.9 | 18, 17, 33 | ||
| 150 µg | 20.7 | 3.2 | 0.8 | 22, 17, 23 | ||
| 500 µg | 20.7 | 3.5 | 0.8 | 17, 21, 24 | ||
| 1500 µg | 25 | 3.5 | 0.9 | 23, 23, 29 | ||
| 5000 µg | 18 | 3.5 | 0.7 | 22, 16, 16 | ||
| TA1537 | DMSO | 12.3 | 0.6 | 12, 13, 12 | ||
| Diisoamylphthalate | 5 µg | 14.7 | 2.3 | 1.2 | 12, 16, 16 | |
| 15 µg | 12.3 | 1.2 | 1 | 11, 13, 13 | ||
| 50 µg | 11.7 | 1.2 | 0.9 | 11, 13, 11 | ||
| 150 µg | 12.3 | 0.6 | 1 | 12, 12, 13 | ||
| 500 µg | 11 | 1 | 0.9 | 12, 10, 11 | ||
| 1500 µg | 13 | 3.6 | 1.1 | 10, 17, 12 | ||
| 5000 µg | 13.3 | 2.9 | 1.1 | 10, 15, 15 | ||
| WP2 uvrA | DMSO | 99.7 | 4.2 | 101, 95, 103 | ||
| (pKM101) | Diisoamylphthalate | 5 µg | 100.3 | 7.6 | 1 | 95, 109, 97 |
| 15 µg | 106.3 | 28.4 | 1.1 | 101, 81, 137 | ||
| 50 µg | 106.3 | 9.5 | 1.1 | 106, 116, 97 | ||
| 150 µg | 126.3 | 8.5 | 1.3 | 123, 136, 120 | ||
| 500 µg | 106 | 10.1 | 1.1 | 104, 97, 117 | ||
| 1500 µg | 95.7 | 18.5 | 1 | 117, 86, 84 | ||
| 5000 µg | 91.7 | 3.5 | 0.9 | 92, 88, 95 | ||
| TA98 | 2NF | 2 µg | 251.7 | 11.5 | 7.6 | 263, 240, 252 |
| TA100 | NaN3 | 2 µg | 663 | 31.1 | 4.4 | 656, 636, 697 |
| TA1535 | NaN3 | 2 µg | 543 | 42.8 | 20.4 | 573, 494, 562 |
| TA1537 | AAC | 50 µg | 807 | 36.6 | 65.4 | 832, 765, 824 |
| WP2 uvrA (pKM101) | NQO | 2 µg | 1796.3 | 499 | 18 | 1896, 1255, 2238 |
| Key to Positive Controls | ||||||
| 2NF | 2-Nitrofluorene | |||||
| NaN3 | Sodium azide | |||||
| AAC | 9-Aminoacridine | |||||
| NQO | 4-Nitroquinoline-1-oxide | |||||
| Results obtained in the presence of metabolic activation: test 1 | ||||||
| Study number: REI0008 | ||||||
| Experiment: Test 1 | ||||||
| Assay conditions: Plate incorporation assay | ||||||
| With metabolic activation | ||||||
| Strain | Addition | Concentration per plate | Mean revertants per plate | Standard Deviation | Fold increase relative to vehicle | Individual revertant |
| colony counts | ||||||
| TA98 | DMSO | 37.3 | 2.9 | 34, 39, 39 | ||
| Diisoamylphthalate | 5 µg | 36.3 | 5.1 | 1 | 32, 42, 35 | |
| 15 µg | 40.3 | 4.7 | 1.1 | 44, 42, 35 | ||
| 50 µg | 45 | 1 | 1.2 | 46, 45, 44 | ||
| 150 µg | 38.7 | 4.7 | 1 | 37, 44, 35 | ||
| 500 µg | 35.3 | 4.2 | 0.9 | 32, 34, 40 | ||
| 1500 µg | 36.7 | 3.2 | 1 | 33, 39, 38 | ||
| 5000 µg | 36 | 5.2 | 1 | 42, 33, 33 | ||
| TA100 | DMSO | 189.7 | 9.6 | 188, 200, 181 | ||
| Diisoamylphthalate | 5 µg | 182 | 20.5 | 1 | 183, 202, 161 | |
| 15 µg | 175.7 | 14 | 0.9 | 161, 177, 189 | ||
| 50 µg | 146.7 | 17.5 | 0.8 | 142, 132, 166 | ||
| 150 µg | 167.3 | 19.6 | 0.9 | 165, 149, 188 | ||
| 500 µg | 180 | 16.7 | 0.9 | 198, 177, 165 | ||
| 1500 µg | 160.7 | 14 | 0.8 | 175, 147, 160 | ||
| 5000 µg | 162 | 7.2 | 0.9 | 170, 156, 160 | ||
| TA1535 | DMSO | 28 | 1 | 28, 29, 27 | ||
| Diisoamylphthalate | 5 µg | 28.3 | 2.5 | 1 | 31, 28, 26 | |
| 15 µg | 26.7 | 2.5 | 1 | 29, 27, 24 | ||
| 50 µg | 26.7 | 5.1 | 1 | 31, 28, 21 | ||
| 150 µg | 27.7 | 1.2 | 1 | 29, 27, 27 | ||
| 500 µg | 22.3 | 9 | 0.8 | 31, 23, 13 | ||
| 1500 µg | 24.3 | 5.5 | 0.9 | 18, 28, 27 | ||
| 5000 µg | 20 | 2 | 0.7 | 20, 18, 22 | ||
| TA1537 | DMSO | 32 | 3 | 29, 35, 32 | ||
| Diisoamylphthalate | 5 µg | 26.7 | 1.2 | 0.8 | 26, 28, 26 | |
| 15 µg | 26 | 6.2 | 0.8 | 21, 24, 33 | ||
| 50 µg | 27.3 | 1.5 | 0.9 | 27, 26, 29 | ||
| 150 µg | 27.3 | 6 | 0.9 | 33, 28, 21 | ||
| 500 µg | 25.3 | 4.9 | 0.8 | 31, 22, 23 | ||
| 1500 µg | 27.3 | 4.5 | 0.9 | 32, 23, 27 | ||
| 5000 µg | 25 | 6.1 | 0.8 | 32, 21, 22 | ||
| WP2 uvrA | DMSO | 147 | 2 | 145, 149, 147 | ||
| (pKM101) | Diisoamylphthalate | 5 µg | 139 | 8 | 0.9 | 139, 147, 131 |
| 15 µg | 148.7 | 14.6 | 1 | 155, 159, 132 | ||
| 50 µg | 140.7 | 6.5 | 1 | 147, 134, 141 | ||
| 150 µg | 150 | 19.2 | 1 | 163, 128, 159 | ||
| 500 µg | 109.3 | 6.5 | 0.7 | 109, 103, 116 | ||
| 1500 µg | 117.3 | 7.1 | 0.8 | 111, 125, 116 | ||
| 5000 µg | 107.3 | 16.5 | 0.7 | 89, 121, 112 | ||
| TA98 | B[a]P | 5 µg | 199 | 8 | 5.3 | 207, 191, 199 |
| TA100 | AAN | 5 µg | 1664.3 | 483.9 | 8.8 | 2063, 1804, 1126 |
| TA1535 | AAN | 5 µg | 347 | 21.5 | 12.4 | 368, 325, 348 |
| TA1537 | B[a]P | 5 µg | 121.3 | 19.3 | 3.8 | 132, 99, 133 |
| WP2 uvrA (pKM101) | AAN | 10 µg | 480.7 | 43.1 | 3.3 | 434, 519, 489 |
| Key to Positive Controls | ||||||
| B[a]P | Benzo[a]pyrene | |||||
| AAN | 2-Aminoanthracene | |||||
| Results obtained in the absence of metabolic activation: test 2 | ||||||
| Study number: REI0008 | ||||||
| Experiment: Test 2 | ||||||
| Assay conditions: Pre-incubation assay | ||||||
| Without metabolic activation | ||||||
| Strain | Addition | Concentration per plate | Mean revertants per plate | Standard Deviation | Fold increase relative to vehicle | Individual revertant |
| colony counts | ||||||
| TA98 | DMSO | 33.7 | 1.2 | 33, 33, 35 | ||
| Diisoamylphthalate | 50 µg | 38.7 | 6.5 | 1.1 | 39, 32, 45 | |
| 150 µg | 32.3 | 4.5 | 1 | 32, 37, 28 | ||
| 500 µg | 42 | 3.5 | 1.2 | 44, 44, 38 | ||
| 1500 µg | 38 | 3.6 | 1.1 | 37, 42, 35 | ||
| 5000 µg | 29.3 | 2.5 | 0.9 | 32, 27, 29 | ||
| TA100 | DMSO | 160.3 | 9 | 166, 165, 150 | ||
| Diisoamylphthalate | 50 µg | 147 | 9.2 | 0.9 | 137, 155, 149 | |
| 150 µg | 151 | 9.2 | 0.9 | 141, 159, 153 | ||
| 500 µg | 155.3 | 4.2 | 1 | 154, 160, 152 | ||
| 1500 µg | 132.7 | 34.2 | 0.8 | 169, 101, 128 | ||
| 5000 µg | 133.7 | 5.5 | 0.8 | 134, 128, 139 | ||
| TA1535 | DMSO | 24.7 | 2.1 | 23, 27, 24 | ||
| Diisoamylphthalate | 50 µg | 17 | 2.6 | 0.7 | 15, 20, 16 | |
| 150 µg | 17.7 | 0.6 | 0.7 | 18, 17, 18 | ||
| 500 µg | 19 | 5.2 | 0.8 | 22, 22, 13 | ||
| 1500 µg | 20 | 3 | 0.8 | 17, 23, 20 | ||
| 5000 µg | 17 | 1 | 0.7 | 17, 18, 16 | ||
| TA1537 | DMSO | 10.3 | 0.6 | 11, 10, 10 | ||
| Diisoamylphthalate | 50 µg | 14 | 2.6 | 1.4 | 17, 12, 13 | |
| 150 µg | 10.3 | 2.9 | 1 | 7, 12, 12 | ||
| 500 µg | 13 | 0 | 1.3 | 13, 13, 13 | ||
| 1500 µg | 12.3 | 4.6 | 1.2 | 7, 15, 15 | ||
| 5000 µg | 10.3 | 4.5 | 1 | 15, 10, 6 | ||
| WP2 uvrA | DMSO | 108.7 | 5.5 | 103, 109, 114 | ||
| (pKM101) | Diisoamylphthalate | 50 µg | 113 | 7.5 | 1 | 105, 114, 120 |
| 150 µg | 103.3 | 16.5 | 1 | 87, 120, 103 | ||
| 500 µg | 101.7 | 11.4 | 0.9 | 111, 89, 105 | ||
| 1500 µg | 103 | 6.1 | 0.9 | 99, 100, 110 | ||
| 5000 µg | 105 | 6.6 | 1 | 111, 98, 106 | ||
| TA98 | 2NF | 2 µg | 265 | 12.2 | 7.9 | 259, 279, 257 |
| TA100 | NaN3 | 2 µg | 800.3 | 50.5 | 5 | 742, 830, 829 |
| TA1535 | NaN3 | 2 µg | 667 | 92.4 | 27 | 568, 751, 682 |
| TA1537 | AAC | 50 µg | 1264.3 | 216.1 | 122.4 | 1380, 1398, 1015 |
| WP2 uvrA (pKM101) | NQO | 2 µg | 1836.3 | 332.4 | 16.9 | 1562, 2206, 1741 |
| Key to Positive Controls | ||||||
| 2NF | 2-Nitrofluorene | |||||
| NaN3 | Sodium azide | |||||
| AAC | 9-Aminoacridine | |||||
| NQO | 4-Nitroquinoline-1-oxide | |||||
| Results obtained in the presence of metabolic activation: test 2 | ||||||
| Study number: REI0008 | ||||||
| Experiment: Test 2 | ||||||
| Assay conditions: Pre-incubation assay | ||||||
| With metabolic activation | ||||||
| Strain | Addition | Concentration per plate | Mean revertants per plate | Standard Deviation | Fold increase relative to vehicle | Individual revertant |
| colony counts | ||||||
| TA98 | DMSO | 45 | 5.6 | 39, 50, 46 | ||
| Diisoamylphthalate | 50 µg | 49.7 | 5 | 1.1 | 49, 45, 55 | |
| 150 µg | 57 | 2 | 1.3 | 55, 59, 57 | ||
| 500 µg | 49.7 | 8.6 | 1.1 | 59, 48, 42 | ||
| 1500 µg | 55 | 1 | 1.2 | 55, 56, 54 | ||
| 5000 µg | 45.3 | 15.9 | 1 | 54, 55, 27 | ||
| TA100 | DMSO | 173.3 | 10 | 163, 174, 183 | ||
| Diisoamylphthalate | 50 µg | 188.7 | 12.9 | 1.1 | 198, 174, 194 | |
| 150 µg | 210.7 | 25.4 | 1.2 | 233, 183, 216 | ||
| 500 µg | 188 | 43.9 | 1.1 | 235, 148, 181 | ||
| 1500 µg | 176.3 | 21 | 1 | 175, 156, 198 | ||
| 5000 µg | 179.3 | 24 | 1 | 185, 200, 153 | ||
| TA1535 | DMSO | 25 | 6.2 | 20, 23, 32 | ||
| Diisoamylphthalate | 50 µg | 20.7 | 0.6 | 0.8 | 21, 21, 20 | |
| 150 µg | 21.3 | 3.1 | 0.9 | 22, 24, 18 | ||
| 500 µg | 24.7 | 3.8 | 1 | 22, 29, 23 | ||
| 1500 µg | 23 | 2.6 | 0.9 | 21, 26, 22 | ||
| 5000 µg | 20.3 | 2.1 | 0.8 | 18, 21, 22 | ||
| TA1537 | DMSO | 28.7 | 2.1 | 28, 31, 27 | ||
| Diisoamylphthalate | 50 µg | 29 | 2 | 1 | 31, 27, 29 | |
| 150 µg | 25.3 | 3.8 | 0.9 | 21, 28, 27 | ||
| 500 µg | 25 | 4.6 | 0.9 | 20, 26, 29 | ||
| 1500 µg | 26 | 5 | 0.9 | 21, 26, 31 | ||
| 5000 µg | 24.7 | 3.2 | 0.9 | 27, 21, 26 | ||
| WP2 uvrA | DMSO | 131.3 | 5.1 | 137, 130, 127 | ||
| (pKM101) | Diisoamylphthalate | 50 µg | 110.3 | 11.5 | 0.8 | 117, 97, 117 |
| 150 µg | 110 | 15.7 | 0.8 | 92, 121, 117 | ||
| 500 µg | 89 | 5.6 | 0.7 | 84, 95, 88 | ||
| 1500 µg | 96 | 8.2 | 0.7 | 103, 87, 98 | ||
| 5000 µg | 110 | 9.6 | 0.8 | 117, 99, 114 | ||
| TA98 | B[a]P | 5 µg | 285 | 12.2 | 6.3 | 277, 279, 299 |
| TA100 | AAN | 5 µg | 1913.7 | 129.1 | 11 | 1852, 2062, 1827 |
| TA1535 | AAN | 5 µg | 170 | 5.6 | 6.8 | 176, 165, 169 |
| TA1537 | B[a]P | 5 µg | 142.7 | 14 | 5 | 147, 154, 127 |
| WP2 uvrA (pKM101) | AAN | 10 µg | 417 | 83.4 | 3.2 | 458, 321, 472 |
| Key to Positive Controls | ||||||
| B[a]P | Benzo[a]pyrene | |||||
| AAN | 2-Aminoanthracene | |||||
Applicant's summary and conclusion
- Conclusions:
It is concluded that diisoamyl phthalate showed no evidence of mutagenic activity in this bacterial system under the test conditions employed.
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