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EC number: 246-562-2
CAS number: 25013-15-4
In a mammalian cell gene mutation assay (similar to OECD 490/GLP), mouse lymphoma L5178Y cells cultured in vitro were exposed to vinyl toluene (approx. 99%; p-Vinyl toluene (31.6%) and m-vinyl toluene (68.4%)) in DMSO in 3 trials at concentrations of 12.5, 25, 50, 100 µg/mL; 10, 20, 40, 60, 80 µg/mL and 40, 45, 50, 55, 60, 65 µg/mL without metabolic activation.
In the first trial, the highest non-lethal concentration of vinyl toluene was 50 µg /mL. At this level there was no increase in mutant fraction, but the Relative Total Growth (RTG) remained high (69% ±7%). The remaining two experiments gave statistically significant responses at 60 µg /mL. This was the only significant dose level, even marginally lower doses (e.g., 55 µg g/mL) inducing considerably less toxicity and no significant increases in mutant fraction. The Relative Total Growth was below 10% at 60 µg /mL in both experiments (experiment 2: 5.5±0.5% and experiment 3: 8±1.0%). According to paragraph 67 of OECD 490 “If the maximum concentration is based on cytotoxicity, the highest concentration should aim to achieve between 20 and 10% RTG. The consensus is that care should be taken when interpreting positive results only found between 20 and 10% RTG and a result would not be considered positive if the increase in MF occurred only at or below 10% RTG (if evaluated).” Therefore, the mutagenic effects at 60 µg /mL in experiments 2 and 3 cannot be treated as positive results. The top dose with no cytotoxicity data in the first experiment is 50µg /mL, and there is no increase in mutant frequency compared to controls, therefore the substance is not mutagenic.
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