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EC number: 246-562-2
CAS number: 25013-15-4
In an in vitro skin corrosion in the human epidermal model EpiDerm (OECD 431/GLP), reconstructed human epidermis tissue was exposed to 50 µL of Vinyltoluene (99.66%; 3-Vinyltoluene CAS No. 100-80-1: 64.3 %; 4-Vinyltoluene CAS No. 622-97-9: 35.7 %) for 3 and 60 minutes. Sterile deionised water was used for the negative control and 8N KOH was used for the positive control. After removal of the test substance, the tissues were incubated with MTT for three hours and then extracted with isopropyl alcohol overnight at room temperature with shaking. The OD540 of isopropyl alcohol extracts was measured on a spectrophotometer. Relative cell viability was calculated for each tissue as % of the mean viability of the negative control tissues.
The controls confirmed the validity of the study. The mean absolute OD570 of the 2 negative control tissues was ≥ 0.8 and ≤ 2.8 [1.7940 (3 min) and 1.7560 (60 mins)]. The mean relative tissue viability (% negative control) of the positive control was < 15% [6.7 % ± 0.1 % or 4.0 % ± 0.2 % (3-minute or 60 mins exposure, respectively)]. The colour of the test substance did not interfere with the endpoint. As the test item displayed a slight purple discolouration due to interaction of the test item with the MTT measurement (reduction of MTT), an additional test with freeze-killed tissues had to be performed. The OD correction factor for the 3-minute exposure was 0.0034, while the OD correction factor for the 1-hour exposure was determined as 0.0295.
The average viability of tissues treated by the test item, 1 Vinyltoluene were higher than the threshold values (50 % and 15% respectively): 85.4 % ± 3.7 after a 3-minute exposure period and 63.5 % ± 2.7 after a 1-hour exposure. The test item should be regarded as non-corrosive.
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