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EC number: 246-562-2
CAS number: 25013-15-4
The following signs of systemic toxicity occurred in all 3 male and 3 female animals immediately after end of the 4-hour exposure and lasted for up to test day 3:
Moderately to severely reduced motility and ataxia were noted in all male and female rats until 60 minutes p.a., and slightly reduced motility and ataxia until 3 hours p.a.
Slightly reduced respiratory rates, tonic and clonic convulsions and slight tremor were observed in all 3 male and 2 of 3 female animals until 3 hours p.a. Slight lacrimation was observed for all animals up to 3 hours p.a. One female rat (no. 5 f) revealed a pronounced sedation until 60 minutes p.a. and was sacrificed prematurely 2 hours after end of exposure due to animal welfare reasons.
Slight pilo-erection was noted up to test day 2 (all 3 females) or test day 3 (all 3 males) p.a.
Furthermore, the following sign of systemic toxicity occurred in 1 of 3 female animals in test week 2 p.a.:
Ptosis and corneal oedema was noted in female no. 6 f on test day 8 or 9 and lasted until test day 11 or 13, respectively.
Feasibility test results summary
Cumulative mass of particles
< 1 µm
< 4 µm
5.20 mg/L air
2.06 mg/L air
Limit study test results summary
and standard deviation
Mass median aerodynamic diameter
Geometric standard deviation
Relation of actual to nominal concentration
5.02 ± 0.02
In an acute inhalation toxicity study (OECD 403/GLP), a group of young adult Crl: CD(SD) rats (3/sex) were exposed to a test atmosphere of Vinyltoluene (99.66%; 3-Vinyltoluene CAS No. 100-80-1: 64.3 %; 4-Vinyltoluene CAS No. 622-97-9: 35.7 %) in air (aerosol) for 4 hours (nose only) at the target concentration of 5 mg/L air (limit test). Animals were then observed for 14 days.
LC50 male/female > 5.02 mg/L air.
The determination of the aerosol particle distribution did not reveal the range for MMAD (1 - 4 µm) and GSD (1.5 - 3.0) as recommended in the guideline. The feasibility tests revealed that the MMAD at a concentration of 2 mg/L air (9.768 µm) was even slightly worse compared to a concentration at 5 mg/L air (9.124 µm).The cumulative mass of particles less than 4 µm was approximately 31% at a concentration at 5 mg/L air and only 11% at a concentration at 2 mg/L air. Hence, the use of 5 mg/L air is from a toxicological point of view more suitable than employing 2 mg/L air, as at a concentration of 5 mg/L air the absolute concentration of smaller particles reaching the alveoli is considered to be larger than at 2 mg/L air. The gravimetric (actual) concentration in the limit test was 5.02 ± 0.02 mg/L air. The mean MMAD and GSD were 9.205 μm and 4.913, respectively.
The test item led to mortality/morbidity in only 1 of the 6 animals. One female rat revealed a pronounced sedation until 60 minutes p.a. and was sacrificed prematurely 2 hours after end of exposure due to animal welfare reasons. Systemic toxicity was noted in in form of reduced motility, ataxia, tremor, a reduced respiratory rate, tonic and clonic convulsions, and/or lacrimation immediately after end of exposure up to 3 hours after end of exposure in all 3 of 3 male and up to 3 of 3 female animals. Pilo-erection was noted up to 48 hours after end of exposure. The body weight did not reveal test item-related changes in any of the rats at the end of the study.The macroscopic examination at necropsy did not reveal any changes. Hence, no histopathological examination was performed. No influence on the lung weights was observed.
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