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EC number: 246-562-2
CAS number: 25013-15-4
phase DOC analysis (ppm)
Acclimation day number.
Treatment level 1
Treatment level 2
percentage removal 93.9.
plate counts organisms/ml
the sludge samples as parent compound equivalents
% of applied dose
Based on the above the DT50 is <0.5hrs and
the DT90 is 5 hrs.
study was conducted to determine the biodegradation of the test
substance by activated sludge in a 33 d study consisting of two phases
(acclimation and biodegradation). The sludge was obtained from a
municipal wastewater treatment plant and diluted with tap water to
approximately 2500 mg solids/L (ca. 3.8 E+7 organisms/mg solids). The
test system consisted of 3 L standard tall aeration vessels (resin pots)
and was modelled after a typical domestic sewage treatment plant. All
ground glass joints were treated with sealing wax to preserve the
integrity of the closed system.
the 14 d acclimation period, non-radiolabelled test material was used at
concentrations ranging from 0 to 50 ppm (vessel 1) or 0 to 100 ppm
(vessel 2) following a daily ‘draw and fill’ routine. Daily operations
included determination of the suspended solids in the mixed liquor, pH
adjustment upon refill and measurement of dissolved oxygen levels in the
mixed liquor. Aliquots of the effluent from each of the two controls and
two test chambers were taken for Dissolved Organic Carbon (DOC) analysis
on Days 12, 13 and 14. Aliquots of the sludge from each of the two
controls and two test chambers were taken for microbial analysis on Days
0, 3, 6, 9, 11 and 15 of the acclimation period and at the end of the
Day 15 of acclimation, 14C/13C-labelled test substance was introduced
with the daily influent at 50 and 100 ppm (as organic carbon). Daily
‘draw and fill’ was discontinued at that time. Trapping solutions for
the collection of 14C-CO2 and 14C-labelled volatile organics in effluent
air, and representative aliquots of the mixtures in each test vessel
were taken at 0.5, 3, 6 and 24 h, then on Days 2, 4, 6, 8, 10, 13, 16
and 19. Analysis was conducted using Liquid Scintillation counting.
outcomes of this 33 d two-phase study showed that the inclusion of the
test substance at two rates (50 and 100 ppm, calculated as carbon
equivalents) did not adversely affect the wastewater treatment process
or affect the sludge microflora. The test substance was found to be
eliminated principally as volatile organic carbon and/or C02. After only
0.5 h, test substance concentrations in the sludge were measured to be
less than 40% of the dose rate. Less than 5% of the dosed substance
remained after 19 d. Elimination as volatile compounds peaked by the
second day, then decreased over the remainder of the test period.
on the results obtained, it was postulated that the high rate of loss
from the reaction vessels was due to volatilisation. A subsequent
experiment introducing the test substance into a completely closed
system resulted in 58.8% accountability at test start, with
concentrations decreasing to 2.7% of initial values after 3 h of
aeration. Since only 4.3% was recovered by trapping, the test substance
was assumed to have been adsorbed by the experimental system (Cranor,
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