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EC number: 639-566-4
CAS number: 165184-98-5
OECD Guideline 211, GLP, key study, validity 2:- The 21-day EC10 value for reproduction was 69 µg a.i./L.
study is available to assess the effects of the test substance on the
survival, growth and reproduction of Daphnia magna during a 21
-day exposure period under flow-through test conditions. This study was
performed according to the OECD Guideline 211, the US EPA OPPTS 850.1300
and ASTM E 1193-97, with GLP statement.
(neonates < 24 hours old at initiation) were exposed to a geometric
series of five test concentrations, a negative control (dilution water)
and a solvent control (0.1 mL/L HPLC-grade dimethylformamide (DMF)). Two
replicate test chambers were tested for each treatment and control group. Each
replicate contained two compartments with five daphnids, resulting in a
total of 20 daphnids in
each treatment and control group.
mean measured concentrations test concentrations were 3.8, 12, 25, 63
and 157 µg a.i./L,
which represented 59, 75, 63, 63 and 63% of nominal concentrations,
results of the study were based on the mean measured concentrations.
daphnids were observed daily during the test for mortality, the onset of
reproduction, and clinical signs of toxicity. Following
the onset of reproduction, the numbers of second-generation daphnids
were counted three times per week and at test termination (Day 21). Body
lengths and dry weights of the surviving first-generation daphnids were
measured at the end of the exposure period.
were no statistically significant treatment-related effects on survival,
reproduction or growth at concentrations ≤ 63 µg a.i./L. Growth,
measured as length and dry weight, and reproduction were the most
sensitive biological endpoints measured in this study. Daphnids exposed
to the test substance at a concentration of 157 µg a.i./L had
statistically significant reductions in growth and reproduction in
comparison to the pooled control.
the 21-day EC10 value for adult immobility was 107 µg a.i./L and the
21-day EC10 value for reproduction was 69 µg a.i./L. The 21-day EC50
value for adult immobility and the 21‑day EC50 value for reproduction
were >157 µg a.i./L, the highest concentration tested.
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