1,4-diisopropylbenzene

Endpoint summary

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: older study; not conducted in accordance with current GLP guidelines

Qualifier:

equivalent or similar to

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

1600, 3200 mg/kg

No. of animals per sex per dose:

four

Control animals:

no

Details on study design:

An acute oral toxicity study was conducted with two groups of four male and four female rats. Animals were fasted overnight prior to administration of the test substance. Dose levels were 1600 and 3200 mg/kg. At study start, animals weighed 170 to 226 grams. The test substance was administered neat by stomach tube.

Preliminary study:

Immediately after dosing, all animals appeared slightly weak. Slight to moderate weakness and roughened hair coats were noted at one hour, and slight weakness and rough hair coats were noted at two hours after dosing. By four hours after administration of the test substance, no abnormal clinical signs were observed in either sex at either dose level. No further abnormal clinical signs were observed at any time during the fourteen-day observation period. All animals gained weight during the study. No necropsies were conducted.

Sex:

female

Dose descriptor:

LD50

Effect level:

> 3 200 mg/kg bw

Based on:

test mat.

Sex:

male

Dose descriptor:

LD50

Effect level:

> 3 200 mg/kg bw

Based on:

test mat.

Mortality:

none

Clinical signs:

Immediately after dosing, all animals appeared slightly weak. Slight to moderate weakness and roughened hair coats were noted at one hour, and slight weakness and rough hair coats were noted at two hours after dosing. By four hours after administration of the test substance, no abnormal clinical signs were observed in either sex at either dose level. No further abnormal clinical signs were observed at any time during the fourteen-day observation period.

Body weight:

At study start, animals weighed 170 to 226 grams.

Gross pathology:

No necropsies were conducted

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

Based on the results of this study, the acute oral LD50 for p-diisopropylbenzend was greater than 3200 mg/kg in male and female rats.

Executive summary:

An acute oral toxicity study was conducted with two groups of four male and four female rats. Animals were fasted overnight prior to administration of the test substance. Dose levels were 1600 and 3200 mg/kg. At study start, animals weighed 170 to 226 grams. The test substance was administered neat by stomach tube. Immediately after dosing, all animals appeared slightly weak. Slight to moderate weakness and roughened hair coats were noted at one hour, and slight weakness and rough hair coats were noted at two hours after dosing. By four hours after administration of the test substance, no abnormal clinical signs were observed in either sex at either dose level. No further abnormal clinical signs were observed at any time during the fourteen-day observation period. All animals gained weight during the study. No necropsies were conducted. Based on the results of this study, the acute oral LD50 was greater than 3200 mg/kg in male and female rats. This study was conducted between February 5 and March 1, 1982.

Acute toxicity: via dermal route

Link to relevant study records

Reference

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: older study; not conducted in accordance with current GLP guidelines

Qualifier:

equivalent or similar to

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

no

Test type:

fixed dose procedure

Limit test:

yes

Species:

guinea pig

Strain:

Hartley

Sex:

not specified

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

the abdomens of six guinea pigs were depilated using a depilatory containing barium sulfide, talc, starch, and a commercial laundry detergent, wetted to make a thin paste. Following depilation, the animals were exposed dermally to doses of 1 (one animal), 5 (one animal), 1 0( one animal), or 20(three animals) mL/kg for 24 hours via gauze pads to which the appropriate amount of test substance had been applied. The pads were held in place by impervious rubber dental dam material wrapped around the torso of the animal and secured with rubber cement.

Duration of exposure:

24 hours

Doses:

1, 5, 10, 20 mL/kg

No. of animals per sex per dose:

1 - 3

Control animals:

no

Details on study design:

The skin was evaluated immediately after removal of the wrapping material and one and two weeks later.

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 20 mL/kg bw

Based on:

test mat.

Mortality:

none

Clinical signs:

Immediately after the animals were unwrapped, clinical signsof irritation on all six animals included moderate to severe erythema, moderate to gross edema and some evidence of necrosis. At one week, thin, dried eschars covered the entire area of the application site for all six animals. By termination of the 14-day study, the application sites had small, scattered light eschars, desquamation, alopecia, and light scarring. The severity of the signs was not related to dose. All animals gained weight during the study.

Body weight:

At initiation of the study, the guinea pigs weighed between 475 and 593 grams.

Gross pathology:

Necropsies were not conducted

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

Based upon the results of this test, the acute dermal LD50 of p-diisopropylbenzene was determined to be greater than 20 mL/kg in guinea pigs.

Executive summary:

In this study the abdomens of six guinea pigs were depilated using a depilatory containing barium sulfide, talc, starch, and a commercial laundry detergent, wetted to make a thin paste. Following depilation, the animals were exposed dermally to doses of 1 (one animal), 5 (one animal), 1 0( one animal), or 20(three animals) mL/kg for 24 hours via gauze pads to which the appropriate amount of test substance had been applied. The pads were held in place by impervious rubber dental dam material wrapped around the torso of the animal and secured with rubber cement. At initiation of the study, the guinea pigs weighed between 475 and 593 grams. The skin was evaluated immediately after removal of the wrapping material and one and two weeks later. Immediately after the animals were unwrapped, clinical signs of irritation on all six animals included moderate to severe erythema, moderate to gross edema and some evidence of necrosis. At one week, thin, dried eschars covered the entire area of the application site for all six animals. By termination of the 14-day study, the application sites had small, scattered light eschars, desquamation, alopecia, and light scarring. The severity of the signs was not related to dose. All animals gained weight during the study. The test substance was rated a moderate irritant under the conditions of this study, and the dermal LD50 was greater than 20 mL/kg. There were no indications of dermal absorption as weight gains were similar across dose levels, and no abnormal clinical signs were noted other than irritation at the application site. This study

was conducted between February 3 and March 3, 1982.