Ammonium wolframate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002-10-24 to: 2002-10-30

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Well documented, scientifically sound study performed in accordance with GLP and the Commission Directive 92/69/EEC. The test method was similar to OECD guideline 202.

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Water solubility (under test conditions): 22 g/L (20 °C after 24 h)

Analytical monitoring:

yes

Details on sampling:

no data

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: To produce the only test concentration 105.5 mg of the test substance were added to 1 L of dilution water, then treated in an ultrasonic bath for 1 hour and afterwards stirred for 24 hours on a magnetic stirrer. Finally, undissolved particles of the test substance were removed by filtration using a folded filter of pore size 7-12 um.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water Flea
- Source: Bundesgesundheitsamt, Berlin

ACCLIMATION
- Acclimation period: A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility.
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: Unicellular green algae (Desmodesmus subspicatus)
- Feeding frequency: ad libitum
- Health during acclimation (any mortality observed): Mortalities of parent Daphnia during the culture period are recorded daily in a semi-quantitative way. The neonates are separated from their parent Daphnia by filtration prior to the acute test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

273.1 mg/L CaCO3

Test temperature:

mean +/- 1 °C (18-22 °C)

pH:

7.7

Dissolved oxygen:

8.8 mg/L

Salinity:

n/a

Nominal and measured concentrations:

Nominal concentration: 100 mg/L
Measured (WO3): 0-hr (7.8 mg/L); 48-hr (3.5 mg/L)
Measured (W): 0-hr (6.2 mg/L); 48-hr (2.8 mg/L)

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Material, size, headspace, fill volume: 50 mL holding 10 neonates in 20 mL of test medium.
- No. of organisms per vessel: 10 neonates
- No. of vessels per concentration (replicates): 2 replicates
- No. of vessels per control (replicates): 2 replicates
- No. of vessels per vehicle control (replicates): 2 replicates

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark
- Light intensity: > 1000 lux

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 4.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

other: dissolved tungsten ion concentration

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 5.7 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

other: tungsten trioxide concentration

Basis for effect:

mobility

Details on results:

No adverse effects were observed in either the control group or the test group for the duration of the test.

Results are expressed in terms of mean measured concentrations. Measured concentrations corresponded to 8.7% of nominal values of tungsten at 0 hours, and to 3.9% of nominal values of tungsten at 48 hours, respectively.

Results with reference substance (positive control):

n/a

Reported statistics and error estimates:

no data

Chemical analysis of the test substance demonstrated that 100 mg of the test substance contained 71 mg tungsten.

Conclusions:

No adverse effects were seen during the duration of the test up to the saturation concentration of the test substance.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

2008-10-14 to: 2009-03-13

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Well documented, scientifically sound study that was conducted in accordance to GLP and OECD guideline 202 with no deviation to the protocol. The reliability of this study for the substance tested is a K1, but in application of read-across to a different substance ECHA’s guidance specifies that the score can be a maximum of K2. Due to similar or lower transformation/dissolution results for the target substance than the source substance, the resulting toxicity potential would also be expected to be similar or lower, so read-across is appropriate. In addition, read-across is justified because the classification and labelling is the same or less severe for the target substance PBT/vPvB profile is the same. Finally, the dose descriptors are, or are expected to be, sufficiently similar or higher for the target substance, and read-across to the source chemical is adequately protective. For more details refer to the attached description of the read across approach on Annex 3 in the CSR.

Justification for type of information:

1. HYPOTHESIS FOR THE CATEGORY APPROACH: The hypothesis is that properties are likely to be similar or follow a similar pattern because of the presence of a common metal ion, in this case tungstate.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES):
Source: Sodium tungstate
Target: Ammonium paratungstate
3. CATEGORY APPROACH JUSTIFICATION: See Annex 3 in CSR
4. DATA MATRIX: See Annex 3 in CSR

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no data

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 10 mL from each control and treatment at 0 and 48 h and transferred to a culture tube.
- Sampling method: 0.05 mL aliquot of each sample was taken, diluted if necessary with 2% HNO3, capped and shaken to mix and analyzed by ICP MS.
- Sample storage conditions before analysis: Room temperature

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.4004 g sodium tungstate dihydrate was dissolved in 2 L aged-blended freshwater. Test concentrations were diluted from this solution.
- Controls: Aged-blended freshwater only.
- Evidence of undissolved material (eg precipitate, surface film, etc): None

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Source: In-house daphnid culture
- Age at study initiation (mean and range, SD): <24h
- Feeding during test
- Frequency: Not fed during test


ACCLIMATION
- Acclimation period: None necessary, as culture conditions are the same as test conditions
- Type and amount of food: Suspension of Pseudokirchneriella subcapitata, supplemented by artificial diet.
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): No mortality in adults at least 48 h prior to testing

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

144 mg/L CaCO3

Test temperature:

19.3-19.5 °C

pH:

8.3-8.5 SU

Dissolved oxygen:

7.6-7.9 mg/L (85 to 89% saturation)

Salinity:

n/a

Nominal and measured concentrations:

Nominal- 0, 13, 25, 50, 100 and 200 mg/L
Mean Calculated- < MQL, 11.9, 21.5, 41.5, 90.8, and 163 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: Glass 250 mL jars, containing 200 mL test solution, covered with a plastic Petri dish
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ABC well water mixed with well water demineralized by reverse osmosis, then aged in a tank containing aquaticorganisms. Water was then passed through a sediment filter, a UV sterilizer, and aerated prior to use.
- Metals: B-0.415 mg/L, Ca-83.1 mg/L, Mg- 33.1 mg/L, K-7.93 mg/L, Na-32.2 mg/L; all others below Detection Limits (DL).
- Pesticides: Below DL
- Alkalinity: 142 mg/L CaCO3
- Conductivity:347 uS
- Culture medium different from test medium: No
- Intervals of water quality measurement: Daily

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hr light, 8hr dark
- Light intensity: 448 lux as measured with a LI-COR Model LI-189 light meter equipped with a photometric sensor.
- The jars were maintained at 20 ± 2 °C in a temperature-controlled water bath.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility and mortality, 24 hr.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2x
- Range finding study
- Test concentrations: 0, 0.10, 1.0, 10, and 100 mg/L
- Results used to determine the conditions for the definitive study: Yes

Reference substance (positive control):

no

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 163 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

other: sodium tungstate concentration

Basis for effect:

other: immobilization

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 163 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

other: sodium tungstate concentration

Basis for effect:

other: immobilization

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

90.8 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

other: sodium tungstate concentration

Basis for effect:

other: immobilization

Details on results:

Biological Results:
After 48 hours of exposure, immobility was 0, 0, 0, 0, 5 and 15% in the 163 mg /L, the highest level tested. Immobility was significantly increased at only the highest test concnetration level. The 48-hour no-observed-effect concentration (NOEC) was determined to be 90.8 mg/L, based on the lack of statistically significant (p=0.05) immobility and sublethal effects at this test substance treatment.

Results with reference substance (positive control):

n/a

Reported statistics and error estimates:

All statistical analyses were performed with SAS software. Estimates of LC50 values and their 95% confidence limits were calculated using the probit method and Trimmed Spearman-Karber method. When the p value for Goodness of Fit was >0.05 and there was no other evidence of questionable convergence, the probit method was selected for reporting. When this criterion was not achieved, the Trimmed Spearman-Karber method was selected for reporting. The no-observed-effect concentration (NOEC) was determined by using a one-tailed Dunnett’s test.

Conclusions:

The 48 hour EC50 value was estimated to be >163 mg/L. The 48-hour no-observed-effect concentration (NOEC) was 90.8 mg/L based on the lack ofstatistically significant (p=0.05) immobility and sublethal effects at this and lower test substance treatments.

Executive summary:

No short-term toxicity to aquatic invertebrates data of sufficient quality were available specifically on ammonium paratungstate (target substance). However, a short-term toxicity to aquatic invertebrates study is available on sodium tungstate (source substance), which are used for read-across.

Due to similar water solubility and lower toxicity for the target substance compared to the source substance, the resulting read-across from the source substance to the target substance is appropriate as a conservative estimate of potential toxicity for this endpoint. In addition, read-across is appropriate because the classification and labelling is more protective for the source substance than the target substance, the PBT/vPvB profile is the same, and the dose descriptors are, or are expected to be, lower for the source substance. For more details, refer to the read-across category approach description in the Category section of this IUCLID submission or Annex 3 of the CSR.

Description of key information

In a 48-hr static test on ammonium paratungstate with Daphnia, no toxic effects up to the saturation concentration under exposure conditions were observed, therefore an EC50 could not be derived. Furthermore, in a test using Daphnia and testing another soluble tungsten compound with the same classification and PBT profile (sodium tungstate) the EC50 observed was found to be greater than the highest concentration tested (>163 mg sodium tungstate/L or approximately 96 mg W/L).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

96 mg/L

Additional information

No short-term toxicity to aquatic invertebrates data of sufficient quality were available specifically on ammonium paratungstate (target substance). However, a short-term toxicity to aquatic invertebrates study is available on sodium tungstate (source substance), which are used for read-across. Due to similar water solubility and lower toxicity for the target substance compared to the source substance, the resulting read-across from the source substance to the target substance is appropriate as a conservative estimate of potential toxicity for this endpoint. In addition, read-across is appropriate because the classification and labelling is more protective for the source substance than the target substance, the PBT/vPvB profile is the same, and the dose descriptors are, or are expected to be, conservative for the target substance. For more details, refer to the read-across category approach description in the Category section of this IUCLID submission or Annex 3 of the CSR.