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EC number: 203-618-0
CAS number: 108-80-5
Table 1: 14CO2
evolution from 14C labelled cyanuric acid on soils
Days at room temp
14C added evolved as CO2 (%)
Chemical plant area
a air-dried in laboratory
for > 6 months. 20 g of H2O was added per 100 g of
air dried soil.
air-dried in laboratory for > 6 months. 20
g of H2O was added per 100 g of air dried soil, experiment started
one month after moistening
c air-dried in laboratory
for > 6 months. 90 g of H2O was added per 100 g of
air dried soil.
Table 1: Percent of 14C
recovered as 14CO2
from 2.5 ppm cyanuric acid
Time after treatment (days)
Aerobic soil plus bean straw
Saturated soil plus bean straw
N.S - not sampled
The carbon of cyanuric acid was rapidly and almost
completely evolved as CO2
with or without organic amendment additions. Even though saturated soil
conditions retarded CO2
evolution, decomposition exceeded 83% in 66 days. The almost complete
evolution of the cyanuric acid carbon to CO2
indicates that the soil population does not use the ring carbon of this
compound for cell synthesis.
At the termination of the experiments the soil waas dried
and the amount of 14C
remaining in the soil was determined. In both the
aerobic and saturated soil incubations the amount recovered as 14CO2
plus the activity remaining in the soil accounted for 91 to 103% of the
initial activity added to the soil.
Table 2: Influence of cyanuric acid on the growth and CO2
evolution by Stachybotrys chartarum and Hendersonula
Growth and CO2evolution
Dry weight of pads
mg per 100 ml of medium
mg C as CO2per100 ml of medium
* Eight week incubation. Cyanuric acid added at the rate
of 166.7 ppm
Cyanuric acid increased CO2
evolution by an amount greater than could be accounted for by the
additional carbon added to the medium as cyanuric acid (166.7 ppm). This
effect probably represents a shift in the metabolism of the organisms.
Table 3: Distribution of 14C
activity in CO2,
fungal cells and fungal products after 8 week incubation of Hendersonula
toruloidea and Stachybotrys chartarum with 166.7 ppm cyanuric
Percent activity recovered as
Humic type polymer
Cyanuric acid was rapidly and completely oxidized to CO2
by the fungus S.
essentially all of the added activity recovered as 14CO2
after 28 days of incubation. No activity could be found in the cell
material and < 4% was recovered in the humic type polymer. The fungus H.
toruloidea exhibited a different pattern of cyanuric acid oxidation.
For the first 28 days of incubation < 1% of the activity was recovered
In the last 28 days of the 8 week incubation, 15% of the activity was
recovered as 14CO2.
THus all of the oxidation of cyanuric acid by this fungus occurred
during the stationary and death phase of growth.
CYA biodegrades readily in soils.
In a series of studies performed in different anaerobic soils
(Saldick J 1974) it was observed that CYA biodegrades readily in
anaerobic soils. Over a 23 day period degradation was highest when there
is a large water to solid ratio and a potentially large anaerobic
microorganism population, for example 100% degradation in farm soil in
In a further study (Wolf & Martin 1975) the relative degradation
rate of cyanuric acid was studied in Greenfield sandy loam soil. After
16 days 87% of the labelled cyanuric acid had evolved as 14CO2
and after 32 days the percentage had increased to 96% indicating that
after ring cleavage the cyanuric acid C is not used for cell synthesis
by the soil organisms. Evolution of 14CO2 was retarded under
saturated soil conditions. Losses were 83% for cyanuric acid in 66 days.
Pure culture studies with two soil fungi, Stachybotrys chartarum and
Hendersonula toruloidea could degrade cyanuric acid to CO2.
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