2-phosphonobutane-1,2,4-tricarboxylic acid

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1993-11-03 to 1993-11-24

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

other: "Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" (proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991)

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

yes

Remarks:

the light intensity was slightly too high (25 instead of max. 20 µE/m2*s). TOC/ COD was not determined. CSB was determined.

Principles of method if other than guideline:

Compared with OECD 211, adopted in 2008): the light intensity was slightly too high (25 instead of max. 20 µE/m2*s).
TOC/ COD was not determined.
CSB was determined.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

The test concentrations were analysed at t= 0h at the start of the experiment (1993-11-03) and after 48h after start of the experiment.
After renewal of the medium at the third day (1993-11-05) of the experiment the test concentrations were analysed at t= 0h and after 72h after renewal of the medium.

Vehicle:

no

Details on test solutions:

NA

Test organisms (species):

Daphnia magna

Details on test organisms:

- Daphnia magna Straus
- parthenogenetic females- Source: West-Aquarium (Bad Lauterberg; Germany)
- Strain: clone breeding of the Federal Health Office, Berlin
- Source: Laborzucht der Bayer AG, Leverkusen (in-house breeding, Bayer AG)
- Age: 6 - 24 hours

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Post exposure observation period:

NA

Hardness:

Not indicated

Test temperature:

ca. 20°C +/-2°C; per single test: 20°C +/-1°C

pH:

Controls: 7.6 - 8.1
1000 mg/L: 7.9-8.6

Dissolved oxygen:

Test vessels containing controls (%): 98.5-121.2
Test vessels containing 1000 mg/L (%): 100.2-129.1

Salinity:

Not indicated

Nominal and measured concentrations:

Nominal concentrations in mg/L.: 10; 31.6; 100; 316; 1000
Measured concentrations in mg/L: 10-11; 31-34; 99-109; 318-337; 991-1159

Details on test conditions:

TEST SYSTEM
- Photoperiod (hrs dark / hrs light): 8 / 16 hours rhythm
- Light intensity: ca.25 µE/m2*s; 1700 Lux
- Test medium:100 mL, non-aerated
- Vehicle: no
No. of organisms per vessel:
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
Feed: algae "Scenedesmus subspicatus"

Reference substance (positive control):

no

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 071 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

immobilisation

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 329 - < 1 071 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

104 mg/L

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: DUNNETT-TEST

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

329 mg/L

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: DUNNETT-TEST

Details on results:

NA

Results with reference substance (positive control):

NA

Reported statistics and error estimates:

The DUNETT test was performed for NOEC (reproduction) and LOEC (reproduction).

No remarks.

Validity criteria fulfilled:

yes

Conclusions:

EC 50 (immobilisation, 21d): > 1071 mg/L
EC 50 (reproduction, 21d): > 329 - < 1071 mg/L
NOEC (reproduction, 21d): 104 mg/L
LOEC (reproduction, 21d): 329 mg/L

Executive summary:

A prolonged toxicity study in Daphnia magna was performed over 21 days.

The respective effects on reproduction (NOEC, LOEC, EC50) and mobility were determined (EC50):

EC 50 (immobilisation, 21d): > 1071 mg/L

EC 50 (reproduction, 21d): > 329 - < 1071 mg/L

NOEC (reproduction, 21d): 104 mg/L

LOEC (reproduction, 21d): 329 mg/L

Description of key information

A prolonged toxicity study in Daphnia magna was performed over 21 days.

The respective effects on reproduction (NOEC, LOEC, EC50) and mobility were determined (EC50):

EC 50 (immobilisation, 21d): > 1071 mg/L

EC 50 (reproduction, 21d): > 329 - < 1071 mg/L

NOEC (reproduction, 21d): 104 mg/L

LOEC (reproduction, 21d): 329 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

NOEC

Remarks:

reproduction

Effect concentration:

> 104 mg/L