Diarsenic trioxide

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Cross-reference

Reason / purpose for cross-reference:

data waiving: supporting information

Data source

Reference

Reference Type:

publication

Title:

The acute toxicity of pesticides to rats

Author:

Gaines, T.B.

Year:

1960

Bibliographic source:

Toxicology and Applied Pharmacology 2, 88-99

Report date:

1960

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: No guideline was followed

GLP compliance:

not specified

Test type:

other: single dermal dose

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Calcium arsenate

EC Number:

231-904-5

EC Name:

Calcium arsenate

Cas Number:

7778-44-1

Molecular formula:

AsH3O4.3/2Ca

Constituent 2

Reference substance name:

Trilead diarsenate

EC Number:

222-979-5

EC Name:

Trilead diarsenate

Cas Number:

3687-31-8

Molecular formula:

AsH3O4.3/2Pb

Specific details on test material used for the study:

Calcium arsenate and lead arsenate

Test animals

Species:

rat

Strain:

Sherman

Sex:

female

Details on test animals or test system and environmental conditions:

The rats used in these studies were reared in a separate building of the laboratory where the tests were made. They were at least 90 days old and had minimum body weights of 175 and 200 g for the males and females, respectively. The animals were individually caged during the study and fed Purina Laboratory Chow. None of them was fasted prior to dosage. The survivors were held for daily observation until they appeared to have recovered completely or for a minimum of 14 days.

Administration / exposure

Type of coverage:

open

Vehicle:

other: 95% ethyl alcohol

Details on dermal exposure:

In this study the test substances were suspended in 95% ethyl alcohol prior testing. The fur of the rats was clipped over the top of the shoulder and forward part of the back with an Ang-Ra No. 2 animal clipper to provide a clean area of about 3.0 X 4.5 cm for application of the test substance. The clipped area of unbroken skin was bathed with a 1:1 solution of acetone and 95% ethyl alcohol to remove dirt and excess oils.
The formulations of the test substances were applied slowly to prevent run-off, using a 1-ml pipette. The rats were observed at least once each hour during the first day after dosage, and twice a day thereafter, for symptoms of poisoning and time of death. The LD50 values were determined by the method of Litchfield and Wilcoson (1949).

Duration of exposure:

14 d

Doses:

0.0016 mL/kg bw

No. of animals per sex per dose:

20 females

Control animals:

not specified

Results and discussion

Effect levels

Effect levels 1

Key result

Dose descriptor:

LD50

Effect level:

> 2 400 mg/kg bw

Based on:

test mat.

Remarks on result:

other: calcium arsenate

Effect levels 2

Key result

Dose descriptor:

LD50

Effect level:

2 400 mg/kg bw

Based on:

test mat.

Remarks on result:

other: lead arsenate

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Conclusions:

Under the study conditions, the LD50s of the tested substances were > 2400 mg/kg bw after single dermal application.

Executive summary:

A 14-day acute dermal toxicity study was conducted to determine the potential toxic effects of calcium arsenate and lead arsenate when applied as a single dermal dose to adult rats. 20 Sherman strain female rats were used. The substances were suspended in 95% ethyl alcohol and tested separately at the dose of 0.0016 mL/kg bw. Under the study conditions, the LD50s of the tested substances were > 2400 mg/kg bw after single dermal application (Gaines, 1960).