Bis(4-chlorophenyl) sulphone

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP compliant study, similar to test guideline OECD 401.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: OECD 401

Deviations:

yes

Remarks:

(i.p. administration, 7-days observation period, no necropsies)

GLP compliance:

yes

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Bis(4-chlorophenyl) sulphone

EC Number:

201-247-9

EC Name:

Bis(4-chlorophenyl) sulphone

Cas Number:

80-07-9

Molecular formula:

C12H8Cl2O2S

IUPAC Name:

1-chloro-4-(4-chlorobenzenesulfonyl)benzene

Details on test material:

- Name of test material: 4,4'-dichlorodiphenylsulfone (DCDPS)
- Physical state: white powder
- Storage condition: at room temperature, protected from light
- Test item sample received on 02-Jan-1989

Test animals

Species:

mouse

Strain:

ICR

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Harlan Sprague Dawley Inc, Frederick, MD, USA
- Age at study initiation: 6-8 weeks old
- Weight at study initiation: males 27-37 g, females 22-28 g
- Housing: in groups of max. 5 animals/cage, in autoclavable plastic cages with filter tops
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature: 74 ±6 °F (23.3 ± 3.4 °C)
- Humidity: 50 ± 20 %
- Photoperiod: 12 hours dark / 12 hours light

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

corn oil

Details on exposure:

Single intraperitoneal injection

Doses:

500, 1000, 1500, 2000, 2500 or 5000 mg/kg bw

No. of animals per sex per dose:

5 animals

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 7 days
- Weighing: once prior to dosing and on days 1 and 3
- Necropsy of survivors performed: no

Statistics:

Probit analysis

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

2 448 mg/kg bw

Based on:

test mat.

Mortality:

The mortality incidences noted in males (m) and females (f) during the 7-days observation period were as follows:
0/5 (m), 0/5 (f) in controls and at 500 and 1000 mg/kg bw
1/5 (m), 2/5 (f) at 1500 mg/kg bw
3/4 (m), 2/5 (f) at 2000 mg/kg bw
5/5 (m), 4/5 (f) at 2500 mg/kg bw
4/5 (m), 4/5 (f) at 5000 mg/kg bw

Clinical signs:

Head tilt, running in circles, lethargy, piloerection, hunched posture and/or cloudy eyes were noted at 1500 mg/kg bw and/or at higher dose levels.

Body weight:

In treated mice, dose-dependent decreases in body weight were noted as compared to pre-treatment body weights.

Gross pathology:

Not determined

Applicant's summary and conclusion

Executive summary:

This acute toxicity study (Microbiological Associates Inc., 1991) was performed as a preliminary test in order to set dose levels for a micronucleus test (see section 7.6.2).

Young adult ICR mice (5 mice/sex/dose level) were treated by intraperitoneal injection of DCDPS in corn oil at dose levels of 0 (vehicle control), 500, 1000, 1500, 2000, 2500 or 5000 mg/kg bw. Dose-dependant mortality was noted in both sexes at 1500 to 5000 mg/kg bw during the 7 -days observation period. Male and female animals treated at 1500 mg/kg bw or higher dose levels showed head tilt, running in circles, lethargy, piloerection, hunched posture and or cloudy eyes. Dose-dependent decreases in body weight were noted in treated mice as compared to pre-treatment body weights.

The LD50 value for the intraperitoneal route was 2448 mg/kg bw. Based on this LD50 value, DCDPS dislays low acute toxicity following intraperitoneal exposure.