(7-methoxynaphthalen-1-yl)acetonitrile

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Version / remarks:

and EU Method B.3 (Acute Toxicity (Dermal))

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Breeder: Janvier, Le Genest-Saint-Isle, France
- Age at study initiation: 8 weeks
- Weight at study initiation: males: 329 ± 4 g
females: 215 ± 3 g
- Fasting period before study: overnight
- Housing: one to seven of the same sex / cage during acclimation period and 3 rats individual housing
during the treatment period in polycarbonate cages with stainless steel lid (48 x 27 x 20 cm)
- Bedding: autoclaved sawdust (by SICSA, France)
- Diet (e.g. ad libitum): ad libitum, pellets by SSNIFF Spezialdiäten GmbH, Germany
- Water (e.g. ad libitum): ad libitum, drinking water, filtered
- Acclimation period: 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2 C
- Humidity (%): 30-70 %
- Air changes (per hr): 12 / hour (filtered, non-recycled air)
- Photoperiod (hrs dark / hrs light): 12/12 h

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: dorsal
- % coverage: 10
- Type of wrap if used: hydrophilic gauze pad, held on with an adhesive hypoallergenic aerated semi-occlusive dressing
and restraining bandage.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): removal of residual test item: by using a dry cotton pad (no washing)
- Time after start of exposure: 24 hours


TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg / kg in its original form in a single dose
- Constant volume or concentration used: yes
- For solids, paste formed: moistened with purified water


VEHICLE
- Amount(s) applied (volume or weight with unit): no vehicle

Duration of exposure:

24 hours

Doses:

2000 mg / kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: frequently during the hours following treatment. At least once a day thereafter.
and weighing: on days 1, 8 and 15
- Necropsy of survivors performed: yes (macroscopic examinations)
- Other examinations performed: local reactions

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

No mortality

Clinical signs:

No clinical signs. No cutaneous reactions.

Body weight:

Slightly reduced in females.

Gross pathology:

No abnormalities.

Other findings:

-

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU