Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study according to OECD guideline 209 under GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Physical state: solid
- Analytical purity: >99%

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- based on pre-experiment, the substance was mixed into deionized water by ultrasonic treatment ober 15 minutes and intensly stirred over 48 hours at room temperature in the dark. For all concentrations, the main part of the test substance was suspended in the test media, so that they were clearly above the water solubility limit of LP-R-6856

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
20°C
pH:
7.4 - 8.5
Dissolved oxygen:
>= 7.2 mg/l
Details on test conditions:
Test concentrations: 10, 32, 100, 320, 1000 mg/l
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
> 1 000 mg/L

Any other information on results incl. tables

Since neither any classification criteria according to GHS/CLP (Regulation (EC) No. 1272/2008) nor any criteria according to DSD (Directive 67/548/EEC) are fulfilled, the substance is not classified for environmental hazards.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
As after 3 hours no effects on the respiration rate were observed, the EC20 and EC50 are clearly higher than the limit of water solubility of the test item or higher than 1000 mg/l nominal.
Executive summary:

As after 3 hours no effects on the respiration rate were observed, the EC20 and EC50 are clearly higher than the limit of water solubility of the test item or higher than 1000 mg/l nominal.

The test was valid as the oxygen consumption of controls at the start and the end differed only by 6.4% and the 3 -hour EC50 of the psoitice control was 27 mg/l. Both values are within the respective guideline-recommended ranges.