1,4-diazabicyclooctane

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study pre-dates GLPs Test material purity was not documented.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: Evaluation and the scoring of the results was similar to that described in Section 1500.40 - Federal Hazardous Substances Act Regulations - 16 CFR - P o 123.

Principles of method if other than guideline:

One group of six (3 male & 3 female) albino rabbits weighing between 2.0 and 3.0 kg. each was employed i n t h i s study. A l l animals had their backs clipped free of hair 24 hours prior to testing. A l l of the animals had their backs abraded prior to dosing.
The animals were housed and maintained in compliance with the Animal Welfare Act (Pub. L-94-279) 9 CFR Part 3.
The sample was dosed as supplied.
The following dosage level was administered:
A l l rabbits were weighed and the correct amount of experimental material was applied to the back of each animal.
These treated areas were covered with large gauze patches and an impervious material was wrapped snugly around the trunk of
each animal. The dressings were removed after twenty-four hours and any excess material was removed and the approximate
amount remaining was noted.
The animals were observed for a 14 day period for signs of toxicity and for mortalities.Gross autopsies were performed on a l l animals which died
during the 14 day observation period and also on a l l survivors of the 14 day observation period.

GLP compliance:

no

Test type:

fixed dose procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4-diazabicyclooctane

EC Number:

205-999-9

EC Name:

1,4-diazabicyclooctane

Cas Number:

280-57-9

Molecular formula:

C6H12N2

IUPAC Name:

1,4-diazabicyclooctane

Test material form:

solid: crystalline

Details on test material:

Dabco Crystalline (lot # 80-D-8)

Test animals

Species:

rabbit

Strain:

other: Albino

Sex:

male/female

Details on test animals or test system and environmental conditions:

The animals were housed and maintained in compliance with the Animal Welfare Act (Pub. L-94-279) 9 CFR Part 3.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

experimental material was applied to the back of each animal.
These treated areas were covered with large gauze patches and an impervious material was wrapped snugly around the trunk of
each animal. The dressings were removed after twenty-four hours and any excess material was removed and the approximate
amount remaining was noted.

Duration of exposure:

24 h

Doses:

2 g/kg

No. of animals per sex per dose:

3

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

No

Clinical signs:

other: Severe erythema lasting for several days.

Gross pathology:

Pathology revealed nothing remarkable.

Any other information on results incl. tables

All animals survived.  Severe erythema which lasted for
several days was the only finding of note.  Gross necropsy
did not reveal anything remarkable.

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

LD50 dermal is > 2000 mg/kg for rabbit.