1,2,4-trimethylbenzene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non-guideline study, available as unpublished report, restrictions in design and/or reporting but otherwise adequate for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

2 rats/sex exposed (whole body) for 4 hours to atmospheres of Shellsol A and observed for the following 14 days

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Solvent naphtha (petroleum), light arom.

EC Number:

265-199-0

EC Name:

Solvent naphtha (petroleum), light arom.

Cas Number:

64742-95-6

IUPAC Name:

64742-95-6

Constituent 2

Reference substance name:

Shellsol A

IUPAC Name:

Shellsol A

Details on test material:

- Name of test material (as cited in study report): Shellsol A
- Substance type: hydrocarbon solvent (essentially C9 isomers, particularly trimethylbenzenes)
- Physical state: liquid
- Source: Shell Nederland Raffinarderij B.V. refinery, Pernis, Rotterdam, The Netherlands

Test animals

Species:

rat

Strain:

other: CD (COBS)

Sex:

male/female

Details on test animals or test system and environmental conditions:

- Source: Charles River (UK) Ltd., Manston, Kent, UK
- Age at study initiation: 7-8 weeks
- Weight at study initiation: no data
- Housing: no data
- Diet: ad libitum (except during exposure)
- Water: ad libitum (except during exposure)
- Acclimation period: no data

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

whole body

Vehicle:

other: air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: tubular glass chamber (2 rats/sex/chamber)
- Exposure chamber volume: no data
- System of generating atmosphere: Atmospheres generated dynamically by the near saturation of air supplied to the test chamber

TEST ATMOSPHERE
- Brief description of analytical method used: monitored continuously by means of a high temperature total hydrocarbon analyser (calibrated using a gravimetric procedure)

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

10.2 mg/L

No. of animals per sex per dose:

2

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no

Statistics:

not applicable

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

10 200 mg/m³ air

Exp. duration:

4 h

Remarks on result:

other: no mortalities at highest dose tested

Mortality:

no mortalities

Clinical signs:

other: lethargic behaviour

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute (4 hour) inhalation LC50 of Shellsol A to male and female rats was greater than 10,200 mg/m3 (10.2 mg/L)

Executive summary:

Rats were exposed for 4 hours to a near saturated atmosphere of Shellsol A (a mixture of trimethylbenzenes) at 10.2 mg/L. There were no deaths and no adverse signs other than lethargic behaviour; The acute LC50 was greater than 10,200 mg/m3.