Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

Currently viewing:

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Title:
No information
Author:
Anonymous
Year:
2007
Bibliographic source:
IUCLID4 data set, 2007

Materials and methods

Test guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-phenyl-2-imidazoline
EC Number:
213-313-4
EC Name:
2-phenyl-2-imidazoline
Cas Number:
936-49-2
Molecular formula:
C9H10N2
IUPAC Name:
2-phenyl-4,5-dihydro-1H-imidazole

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Metabolic activation system:
Phenobarbital / beta-naphthoflavone co-induced rat liver S9 fraction
Test concentrations with justification for top dose:
50 to 5000 Ug/plate

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid
Additional information on results:
GENOTOXIC EFFECTS:
- With metabolic activation: None
- Without metabolic activation: None
The positive controls were functional in the finally evaluated tests.
PRECIPITATION CONCENTRATION: Precipitation was not observed.
CYTOTOXIC CONCENTRATION (including effects on background lawn):
TA 98: 5000 Ug/plate (+ S9), 1500 Ug/plate (- S9)
TA 100: none (+ S9), 2000 Ug/plate (- S9)
TA 1535: 5000 Ug/plate (+ S9), >= 2000 Ug/plate (- S9)
TA 1537: 5000 Ug/plate (+ S9), 2000 Ug/plate (- S9)
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion