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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reason / purpose:
read-across source
Key result
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
560 mg/L
Nominal / measured:
nominal
Conc. based on:
other: expressed as solid content
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
other: expressed as solid content
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Validity criteria fulfilled:
yes
Conclusions:
In accordance with OECD 209 (2010) and according to GLP principles the influence of a related member of the chemical category (amphoacetates C12) on the respiration rate of activated sludge was investigated after a contact time of 3 hours. The substance was not toxic to waste water (activated sludge) bacteria at a concentration of 560 mg/L (NOEC). This result is read-across to the registered substance.
Executive summary:

In accordance with OECD 209 the influence of a substance analogue (amphoacetates C12) on the respiration rate of activated sludge was investigated after a contact time of 3 hours. In a combined limit/range-finding test concentrations of 20, 200 and 2000 mg/l were tested. No statistically significant inhibition of the respiration rate of the sludge was recorded at the concentrations 20 and 200 mg/L. At 2000 mg/l, the average inhibition of the respiration was 70%. Therefore, a final test was performed at 100, 180, 320, 560 and 1000 mg/l. No statistically and biologically significant inhibition of the respiration rate of the sludge was recorded in this test at 560 mg/L (NOEC). At 1000 mg/L, the average inhibition of the respiration was 34%. The batch of activated sludge was checked for sensitivity by testing the reference substance 3,5-dichlorophenol, which showed normal sensitivity. The validity criteria were all fulfilled. The results are read-across to the registered substance.

Description of key information

In accordance with OECD 209 (2010) and according to GLP principles the influence of a substance analogue (amphoacetates C12) on the respiration rate of activated sludge was investigated after a contact time of 3 hours. The substance was not toxic to waste water (activated sludge) bacteria at a concentration of 560 mg/L (NOEC). This result is read-across to the registered substance.

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L
EC10 or NOEC for microorganisms:
560 mg/L

Additional information

In accordance with OECD 209 the influence of substance analogue (amphoacetates C12) on the respiration rate of activated sludge was investigated after a contact time of 3 hours. In a combined limit/range-finding test concentrations of 20, 200 and 2000 mg/l were tested. No statistically significant inhibition of the respiration rate of the sludge was recorded at the concentrations 20 and 200 mg/L. At 2000 mg/l, the average inhibition of the respiration was 70%. Therefore, a final test was performed at 100, 180, 320, 560 and 1000 mg/l. No statistically and biologically significant inhibition of the respiration rate of the sludge was recorded in this test at 560 mg/L (NOEC). At 1000 mg/L, the average inhibition of the respiration was 34%. The batch of activated sludge was checked for sensitivity by testing the reference substance 3,5-dichlorophenol, which showed normal sensitivity. The validity criteria were all fulfilled. The results are read-across to the registered substance.