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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1954
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well performed study, study performance before implementation of GLP, purity of test item not reported
Cross-referenceopen allclose all
Reason / purpose:
reference to same study
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Range-finding toxicity data, List V
Author:
Smyth, H.F., Carpenter, C.P., Weil, C.S., Pozzani, U.C.
Year:
1954
Bibliographic source:
Arch. Ind. Hyg. Occup. Med., 10, 61-68

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Principles of method if other than guideline:
not applicable
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
dibutyl ether (purity not indicated)

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: raised at Mellon Institute of Industrial Research
- Age at study initiation: young adults
- Weight at study initiation: 90-120 grams

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
air
Details on inhalation exposure:
The stream was prepared by various styles of proportioning pumps. Nominal concentrations were recorded, not confirmed by analytical methods. Exposures were four hours long. Concentrations were in an essentially logarithmic series with a factor of two.
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
4 h
Concentrations:
other
No. of animals per sex per dose:
6
Control animals:
no
Details on study design:
Groups of six male Carworth-Wistar rats (90 to 120 g), raised at the Mellon Institute of Industrial Research, University of Pittsburgh, USA, were exposed to a flowing stream of known vapor concentrations of dibutyl ether. The stream was prepared by various styles of proportioning pumps. Nominal concentrations were recorded, not confirmed by analytical methods. Exposures were four hours long. Concentrations were in an essentially logarithmic series with a factor of two. The concentration yielding fractional mortality among six rats within 14 days was recorded. Clinical signs were not reported.
Statistics:
no information available

Results and discussion

Preliminary study:
not applicable
Effect levels
Sex:
male
Dose descriptor:
other: approximate LC50
Effect level:
ca. 21 600 mg/m³ air (nominal)
Based on:
test mat.
Exp. duration:
4 h
Remarks on result:
other: approximately 4000 ppm
Mortality:
The 4-hour exposure to 4000 ppm dibutyl ether killed 2/6 rats within 14 days.
Clinical signs:
no information available
Body weight:
no information available
Gross pathology:
no information available
Other findings:
no information available

Any other information on results incl. tables

4000 ppm are at 25 °C and 1013 hPa equal to 21600 mg/m³.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The 4-hour exposure to 4000 ppm dibutyl ether killed 2/6 rats within 14 days. This substance is not classified for Acute toxicity
Executive summary:

In this study, groups of six male Carworth-Wistar rats (90 -120 grams) were exposed to a flowing stream of known vapour concentrations of dibutyl ether for 4 hours. Under the conditions of the study, the 4-hour exposure to 4000 ppm dibutyl ether killed 2/6 rats within 14 days and this substance is not classified for Acute toxicity.