2-benzyl-2-dimethylamino-4'-morpholinobutyrophenone

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: OECD-GUIDELINE No. 301 B) (Paris 1981)

Deviations:

yes

Remarks:

The volume of the test solution was reduced from 3.0 L to 1.5 L.

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Sewage treatment plant of CH - 4153 Reinach, Switzerland
- Pretreatment: According to guideline

Duration of test (contact time):

28 d

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: According to guideline
- Solubilising agent (type and concentration if used): The test substance was added to the medium, homogenized with Nonylphenol 10E05P0
- Test temperature: 22± 2 °C
- pH adjusted: No
- Aeration of dilution water: Yes, 24 hours in the test vessel

TEST SYSTEM
- Culturing apparatus: 2 Liter flasks equipped with gas inlet and magnetic stirrer
- Number of culture flasks/concentration:
- Method used to create aerobic conditions: Air flow, appr. 25 mL/min free of carbon dioxide
- Method used to create anaerobic conditions:
- Details of trap for CO2 and volatile organics if used: Absorbers filled with 200 mL 0.05 N sodium hydroxide

SAMPLING
- Sampling frequency: At day 4, 7, 11, 15, 18, 22, 27, and 28
- Sampling method: Determination of the initial CO2 of the 0.05 N sodium hydroxide and the CO2 absorbed in the absorbers

CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Other: Vehicle control

Reference substance:

aniline

Remarks:

20 mg/L

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

28 d

Remarks on result:

other: 10 mg test substance/L

Parameter:

% degradation (CO2 evolution)

Value:

3

Sampling time:

28 d

Remarks on result:

other: 20 mg test substance/L

Results with reference substance:

Biodegradation 87.2 % in 28 days

Table 1. CO₂ evolution in the controls and in the reference substance cultures

Day	CO2 evol.				CO2 evol.		Biodegrad.
	Blank		Blank + Vehicle		Aniline (20 mg/L)
	(mg)	(mg)*	(mg)	(mg)*	(mg)	(mg)*	(%)	(%)*
4	6	6	7	7	38.5	38.5	45.3	45.3
7	3	9.1	4	11	20.7	59.2	24.3	69.6
11	3.9	12.9	3.6	14.6	7.4	66.6	8.7	78.3
15	3.8	16.8	4.4	18.9	2.4	69	2.8	81.1
18	2.8	19.6	3.1	22	4.4	73.4	5.2	86.3
22	3.3	22.9	3.9	25.9	-1.2	72.2	-1.4	84.9
27	6.9	29.8	5.9	31.9	-0.5	71.7	-0.6	84.3
28	-0.8	29	-0.1	31.7	2.5	74.2	2.9	87.2

* cumulative values

For calculation of the degradation, the Blank+Vehicle is used. The Blank shows the behavior of the inoculum.

Table 2. CO₂ evolution in TK 11319 cultures

Day	Concentration 10 mg/L				Concentration 20 mg/L
	CO2 evol.		Biodegrad.		CO2 evol.		Biodegrad.
	(mg)	(mg)*	(%)	(%)*	(mg)	(mg)*	(%)	(%)*
4	0.2	0.2	0.4	0	2.5	2.5	3	3
7	-1	-0.8	-2.4	-2	-1.2	1.2	-1.5	2
11	-0.1	-1	-0.4	-2	0.4	1.7	0.5	2
15	-0.6	-1.6	-1.5	-4	-0.4	1.3	-0.5	2
18	0.5	-1.1	1.2	-3	0.6	1.9	0.7	2
22	-1.6	-2.6	-3.8	-6	-1.3	0.6	-1.5	1
27	-0.1	-2.8	-0.4	-7	0.7	1.2	0.8	2
28	1.8	-1	4.4	-2	1.4	2.6	1.7	3

* cumulative values

The negative degradation values are within the variation of the method.

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

After 28 days, the substance achieved 3% biodegradation - thus EC 404-363-3 is not readily biodegradable according to OECD criteria.

Executive summary:

In this guideline (OECD 301 B) study conducted with GLP certification, the test material (EC 404-363-3) was determined to not be readily biodegradable. The test was conducted in aerobic conditions, using an adapted sludge sourced from a municipal sewerage treatment plant. 3% test material biodegradation was observed 28 days (CO2 evolution).

Description of key information

Study carried out according to guideline and with GLP compliance.

Additional information

A test on ready biodegradability was conducted according to GLP and OECD 301B (CIBA-GEIGY Ltd. 1989). After 28 d only 3% biodegradation was observed. Therefore, the test substance is poorly biodegradable and not readily biodegradable (according to OECD criteria).

The test substance is not readily biodegradable (according OECD criteria). Poorly biodegradable.