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EC number: 257-406-8
CAS number: 51772-35-1
The potential of the test article to cause ocular irritation was
assessed by a single topical application of 50 μL bulk volume (about 19
mg) of the test substance to a reconstructed three dimensional human
cornea model (EpiOcular™). Two EpiOcular™ tissue samples were incubated
with the test substance for 90 minutes followed by a 18-hours
post-incubation period. Tissue destruction was determined by measuring
the metabolic activity of the tissue after exposure/post-incubation
using a colorimetric test. The reduction of mitochondrial dehydrogenase
activity, measured by reduced formazan production after incubation with
a tetrazolium salt (MTT) was chosen as endpoint. The formazan production
of the testsubstance treated epidermal tissues is compared to that of
negative control tissues. The quotient of the values indicates the
relative tissue viability. The EpiOcular™ eye irritation test showed the
The test substance is not able to reduce MTT directly. The mean
viability of the test-substance treated tissues was 98%. Based on the
observed results it was concluded, that the test substance does not show
an eye irritation potential in the EpiOcular™ eye irritation test under
the test conditions chosen.
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