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EC number: 257-406-8
CAS number: 51772-35-1
viability [% of NC]
The test article's potential to cause dermal irritation was assessed in
an in vitro irritation test according to OECD guideline 439 and in
compliance with GLP. To that end, a single topical application of 25 μL
bulk volume (about 14 mg) of the test substance to a reconstructed three
dimensional human epidermis model (EpiDerm™) was analyzed. Three
EpiDerm™ tissue samples were incubated with the test substance for 1
hour followed by a 42-hours post-incubation period. Tissue destruction
was determined by measuring the metabolic activity of the tissue after
exposure/ post-incubation using a colorimetric test. The reduction of
mitochondrial dehydrogenase activity, measured by reduced formazan
production after incubation with a tetrazolium salt (MTT) was chosen as
endpoint. The formazan production of the testsubstance treated epidermal
tissues is compared to that of negative control tissues. The quotient of
both values indicates the relative tissue viability. The EpiDerm skin
irritation test showed the following results: The test substance is not
able to reduce MTT directly. The mean viability of the test-substance
treated tissues determined after an exposure period of 1 hour with about
42 hours post-incubation was 102%. Based on the observed results it was
concluded, that the test substance does not show a skin irritation
potential in the EpiDerm™ skin irritation test under the test conditions
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