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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, meets national standard methods, limited documentation, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
other: Norme Francais EN 28692
Qualifier:
equivalent or similar to
Guideline:
EU Method C.3 (Algal Inhibition test)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): fluorene
- Molecular formula (if other than submission substance): C13H10
- Molecular weight (if other than submission substance): 166
- Substance type: organic
- Physical state: solid
- Source: no data
- Analytical purity: no data
- Typical concentration in anthracene oil (<50 ppm BaP): 25 - 31 % (R4CC - GC analysis)
- water solubility: ~ 2mg/L
- log Pow = ~4.2
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES (data from NL 2008: Coal-Tar Pitch, Annex-XV dossier, and WHO 1998: EHC 202, if not otherwise indicated)
- Vapour pressure: 8.0 - 8.3*10^-2 Pa (at 25 °C)
- Water solubility: in freshwater, approx. 1800 - 1980 µg/L (20 - 25 °C);
- Henry's law constant: 14.3 Pa*m3/mol (at 25 °C)
- log Pow: = 3.9 - 4.0

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
at 0. 24, 48, and 72 h

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution close to water saturation used for dilution series (no detailed data, see Report p. 34)
- Eluate: no
- Differential loading: no
- Controls: with and without vehicle
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no data
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): no data

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Source (laboratory, culture collection): in-house, origin Culture Centre of Algae and Protozoa, University of Cambridge
- Age of inoculum (at test initiation):
- Method of cultivation: weekly inoculation into fresh culture medium

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Hardness:
approx. 215 mg/L (as CaCO3)
Test temperature:
23 +-2°C
pH:
no data
Nominal and measured concentrations:
nominal: not documented
analytical: Estimation of the EC values from measured concentrations at 0, 24, 48, and 72 h,
based on the degradation constant (see Report, p. 40 - 44).
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Erlenmeyer 250 mL, test volume 100 mL
- Aeration: no
- Initial cells density: approx. 10^4 cells/mL
- Control end cells density:
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 3
- agitation: orbital shaker, 150 rpm


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic mediumprepared from stock solutions of mineral salts (Report., p. 29/30)

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: 6000 - 8000 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter (Coulter Counter) at 24, 48, and 72 h


TEST CONCENTRATIONS
- Spacing factor for test concentrations: no data
- Range finding study no data
Reference substance (positive control):
yes
Remarks:
potassium bichromate K2Cr2O7

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
82 µg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
dissolved
Remarks:
test material
Basis for effect:
other: biomass and growth rate
Remarks on result:
other: Note: in Report, Tab. 11, misprint 820 µg/L (comp. Report Summary, p. 135)
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
485 µg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Remarks:
test material
Basis for effect:
other: biomass and growth rate
Remarks on result:
other: 95% C.I.: 411 - 540 µg/L
Results with reference substance (positive control):
- Results with reference substance valid? yes
- mean ECb50: 0.22 - 0.45 mg/L
- mean ECr50: 0.67 - 1.05 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Adverse effects within the range of water solubility