Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to Read Across Statement attached in Section 13
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 450 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- No further details are available.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: up to 80 % inhibition occurred - Reported statistics and error estimates:
- In order to calculate the inhibition effects of the test item at a particular concentration, the respiration rate was expressed as percentage of the mean of the 2 control respiration rates.
The degree of inhibition (EC10 and EC20) of the test item was evaluated by Probit analysis according to Finney. The values were given with an accuracy of 2 significant digits.
The degree of inhibition (EC20; EC50 and EC80) of the reference item was also evaluated by Probit analysis according to Finney. - Validity criteria fulfilled:
- yes
- Remarks:
- Validity criteria of the applied guideline were fulfilled.
- Conclusions:
- The study is regarded as a valid guideline study conducted under certificated GLP compliance. According to the reported EC50 value (> 1000 mg/L, nominal) the test substance is not acutely toxic towards aquatic microorganisms. The same result is expected for the organic constituents of target substance since it has the same composition as the source substance.
- Executive summary:
The toxicity of Acetic acid, oxo-, sodium salt, reaction products with ethylenediamine and phenol, iron sodium salts (CAS 84539-55-9) towards freshwater microorganisms was investigated in accordance to OECD Guideline 209 / EU Method C11. Activated sludge was collected from the aeration tank at a municipal wastewater treatment. Aliquots of the stock solutions with test- or reference substance were pipetted into test vessels and made up with demineralized water to a volume of 150 mL. After that 8 mL synthetic medium were given to the test vessel. To prepare the blank control assays 150 mL of demineralized water and 8 mL synthetic medium were given to the blank control test vessels. The pH-values were measured in all test vessels. An adjustment was not necessary. 42 mL demineralized water were added to each test vessel. After addition of 50 mL of the inoculum suspension (dry weight 7.5 g/L) the incubation was started by aeration of the test vessels with pressure air. The vessels for the blank control assays were prepared according to the same procedure without addition of test- or reference item. After 180 minutes incubation the mixtures in the test vessels were placed subsequently into a closed oxygen measuring cell. As a result, an EC50(3h) and EC80(3h) > 1000 mg/L and an EC10(3h): 450 mg/L is reported.
- Endpoint:
- activated sludge nitrification inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Please refer to Read Across Statement attached in Section 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.33 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Remarks:
- converted to target substance
- Basis for effect:
- inhibition of nitrification rate
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- 4.67 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Remarks:
- converted to target substance
- Basis for effect:
- inhibition of nitrification rate
- Duration:
- 3 h
- Dose descriptor:
- other: IC20
- Effect conc.:
- 2.13 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Remarks:
- converted to target substance
- Basis for effect:
- inhibition of nitrification rate
- Conclusions:
- The nitrite and nitrate production in nitrification decreases as the concentration of heavy metals increases. Consequently, the % inhibition increases as the concentration of heavy metals increases. The no observed effect concentration is 0.1 mg Zn/L, the IC20 is reached at 0.16 mg/L Zn2+ and the IC50 is reached at 0.35 mg/L Zn2+.
As the target substance contains 7.5 % Zinc ion the no observed effect concentration for the target substance is 1.33 mg/L, the IC20 is reached at 2.13 mg/L and the IC50 is reached at 4.67 mg/L. - Executive summary:
In this study the ISO 9509 test was used to assess the inhibitory effect of Zn2+ ion nitrification of municipal wastewater samples using activated sludge collected from a municipal wastewater treatment plant. The nitrite and nitrate production in nitrification decreases as the concentration of heavy metals increases. The no observed effect concentration is 0.1 mg Zn/L, the IC20 is reached at 0.16 mg/L Zn2+ and the IC50 is reached at 0.35 mg/L Zn2+.
This result is also relevant for the target substance, which contains 7.5 % zinc ions. Accounting to the Zn content of 7.5 %, this corresponds to a no observed effect concentration of 1.33 mg/L, an IC20 of 2.13 mg/L and an IC50 of 4.67 mg/L.
Referenceopen allclose all
Measured data of oxygen content of the test assays at the begin and end of the evaluation time:
Assay identification |
BC1 |
BC2 |
RS1 |
RS2 |
RS3 |
TS1 |
TS2 |
TS3 |
TS4 |
TS5 |
O2-concencentration Start [mg/L] |
8.0 |
8.0 |
8.4 |
8.7 |
9.1 |
8.2 |
8.3 |
8.5 |
7.8 |
7.6 |
O2 -concentration after 6 minutes [mg/L] |
7.0 |
7.0 |
7.5 |
8.2 |
8.9 |
7.5 |
7.5 |
7.5 |
6.5 |
6.0 |
O2consumption rate [mg/L in 6 min] |
1.0 |
1.0 |
0.9 |
0.5 |
0.2 |
0.7 |
0.8 |
1.0 |
1.3 |
1.6 |
O2-consumption rate [mg/Lxh] |
10 |
10 |
9 |
5 |
2 |
7 |
8 |
10 |
13 |
16 |
Specific O2-consumption rate [mg O2/gxh] |
7 |
7 |
6 |
3 |
1 |
5 |
5 |
7 |
9 |
11 |
RS/TSconcentrations[mg/L] |
- |
1 |
10 |
100 |
1000 |
500 |
250 |
125 |
62.4 |
|
Calculation of inhibition respiration[%] |
- |
- |
10 |
50 |
80 |
30 |
20 |
0 |
-30 |
-60 |
Legend: TS = test item, RS = reference item, BC = blank control
The target substance contains 7.5 % Zn ions. Therefore, the hazard value for Zn ion is converted to the target substance considering a Zn content of 7.5 %.
IC50 (Zn ion) = 0.35 mg/L
IC50 (target substance) = 0.35/0.075 = 4.67 mg/L
IC20 (Zn ion) = 0.16 mg/L
IC20 (target substance) = 0.16/0.075 = 2.13 mg/L
NOEC (Zn ion) = 0.1 mg/L
NOEC (target substance) = 0.1/0.075 = 1.33 mg/L
Description of key information
In an activated sludge respiration inhibition test (OECD TG 209, GLP) Fe(Na)EDDHA was used as test substances. An EC50(3 h) value of > 1000 mg/L was obtained (BASF 2010). Hence, the substance can be regarded as non-toxic to microorganisms present in sewage treatment plants.
With regard to zinc the key value for chemical safety assessment for toxicity to microorganisms is obtained in an activated sludge inhibition test (ISO 9509). An IC50 (3 h) of 0.35 mg Zn/L was determined (Juliastuti 2003). Converted to the zinc moiety in the target substance taking into account the maximum amount of zinc in the target substance, this key value corresponds to an IC50 (3 h) of 4.67 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 4.67 mg/L
- EC10 or NOEC for microorganisms:
- 1.33 mg/L
Additional information
Read-across - Zinc Ion
Juliastuti et al., 2003
In this study the ISO 9509 test was used to assess the inhibitory effect of Zn2+ ion nitrification of municipal wastewater samples using activated sludge collected from a municipal wastewater treatment plant. The nitrite and nitrate production in nitrification decreases as the concentration of heavy metals increases. The no observed effect concentration is 0.1 mg Zn/L, the IC20 is reached at 0.16 mg/L Zn2+ and the IC50 is reached at 0.35 mg/L Zn2+. This result is also relevant for the target substance, which contains 7.5 % zinc ions. Accounting to the Zn content of 7.5 %, this corresponds to a no observed effect concentration of 1.33 mg/L, an IC20 of 2.13 mg/L and an IC50 of 4.67 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.