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EC number: 617-769-9 | CAS number: 858956-08-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 Mar - 23 Apr Jun 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Version / remarks:
- 2000
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 217 (Soil Microorganisms: Carbon Transformation Test)
- Version / remarks:
- 2000
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, Energie, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- AMENDMENT OF SOIL
- Type of organic substrate: For the nitrogen transformation the soil was thoroughly mixed with the ground lucerne meal before application. The final concentration of the dried lucerne meal was 0.5% of the soil dry weight.
For the short-term respiration the amount of glucose needed to obtain maximum shortterm rates of respiration in the test soil was determined prior to the beginning of the test. A concentration of 2 g glucose/kg soil wet weight was found to be the optimum and was used in the test.
APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: 20 mg of the test substance was dissolved in 100 mL deionised water. Appropriate amounts of this stock solution were mixed into the soil. Deionised water was added to the soil to reach the desired range of soil moisture, resulting in a final water
content of 45 to 51% of the WHCmax. - Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 20° ± 2°C
- Moisture:
- 45 - 53% of the WHCmax
- Organic carbon content (% dry weight):
- 1.16
- Details on test conditions:
- TEST SYSTEM
- Test container: 500 mL plastic boxes with perforated plastic lids (nitrogen transformation test) / 1000 mL plastic boxes, with perforated plastic lids (short-term respiration test)
- Amount of dry soil: 400 g (nitrogen transformation test) / 800 g (short-term respiration test)
- No. of replicates per concentration: 3
- No. of replicates per control: 3
SOIL INCUBATION
- Method: bulk
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site: longitude 8° 44' 38.70'' E, latitude 49° 51' 59.59'' N
- History of site: For at least four years prior to test initiation, no pesticides had been used on the soil. No organic or mineral fertilizer had been applied to the soils for two years prior to study initiation, respectively.
- Treatments with pesticides or fertilizers: no
- Accidental contamination: no
- Other: sampled (according ISO 10381-6, sampling depth 0.05 to 0.2 m)
- Soil texture
- % sand: 57.5
- % silt: 33.1
- % clay: 9.4
- Soil taxonomic classification: mid loamy sand
- pH (in water): 6.9
- Initial nitrate concentration for nitrogen transformation test: 16.425 mg/kg dry weight
- Maximum water holding capacity (in % dry weigth): 41.6
- Cation exchange capacity (mmol/kg): 46.25 mmol Ba/kg dry weight
- Pretreatment of soil: air dried and sieved (mesh 2 mm) at room temperature, preincubated at 46% of its maximum water hold capacity in ventilated boxes at about 20°C before used in the experiment
- Initial microbial biomass as % of total organic C: 1.55
- Storage (condition, duration): 3 weeks at 20° ± 2°C
EFFECT PARAMETERS MEASURED: nitrogen transformation and soil respiration (days 0, 7 (8), 14, and 28 days following application of the test item) - Nominal and measured concentrations:
- Nominal test substance concentrations: 0.74 and 3.68 mg/kg dw
- Reference substance (positive control):
- yes
- Remarks:
- sodium chloride (tested at least once a year at the test facility)
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- >= 3.68 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks:
- respiration rate
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- >= 3.68 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks:
- respriration rate
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 3.68 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: Difference from control: soil respiration rates: -67.28%, nitrate-N content: -67.74, cumulative nitrate formation rate: -107.79% - Reported statistics and error estimates:
- Data for the short-term respiration and the soil nitrate contents were tested for normality and homogeneity of variance using the R/S-Test Test (α = 0.05) and Cochran´s-Test ( = 0.05). Where the criterion of homogeneity was fulfilled, the Student t-test and in case of inhomogeneity the Welch t-test (pair-wise comparison, two sided, α = 0.05) was used for comparison of treated and control values.
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
Reference
Table 1: Summary of effects of Aminocyclopyrachlor on nitrate formation and short- term respiration in soil
Amino- cyclo- pyra- chlor concen- trationa |
NO3–N levels (Day 28) |
Nitrate formation rate (Day 0 to 28) |
Nitrate formation rate (Day 14 to 28) |
Respiration rate (Day 28) |
||||
mg/kg dry soil |
% Dev. from control bc |
mg/kg dry soil/day |
% Dev. from control bc |
mg/kg dry soil/day |
% Dev. from control bc |
mg CO2/hr /kg dry soil |
% Dev. from controlbc |
|
Control |
31.657 |
--- |
0.53 |
--- |
1.37 |
--- |
8.443 |
--- |
0.74 mg/kg sdw |
29.784 |
-5.92 |
0.49 |
-7.55 |
1.29 |
-5.84 |
8.016* |
-5.06 |
3.68 mg/kg sdw |
28.489 |
-10.01 |
0.45 |
-15.09 |
1.26 |
-8.03 |
7.966* |
-5.65 |
a Test item concentrations correspond to 1- and 5-times the maximum PECsoil for Aminocyclopyrachlor
b Negative value =% inhibition, positive value =% stimulation
c Statistical evaluation (Student t-test + Welch t-test, two-sided, α = 0.05):
*significant differences from the control
Table 3: Variation of Replicate Control samples: Nitrogen and carbon transformation test: nitrate content
|
CV1)% |
|
day |
nitrate content |
soil respiration |
0 |
0.46 |
2.67 |
72) |
14.70 |
2.08 |
14 |
5.58 |
2.48 |
28 |
9.54 |
2.42 |
1) Coefficient of variation
2) Day 8 due to measurement failure on day 7 for soil respiration
Table 2: Validity criteria for OECD 216 and 217
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The variation between replicate control samples should be less than ± 15%. |
0.46 - 14.7 |
yes |
Description of key information
NOEC (28 d) ≥ 3.68 mg/kg soil dw (nominal, OECD 216 & 217, nitrogen & carbon transformation)
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 3.68 mg/kg soil dw
Additional information
The effects of aminocyclopyrachlor on soil microflora were assessed in a test that measured nitrogen transformation and short-term respiration following an application of the test substance to soil. The test was conducted in accordance with the OECD 216 and 217. The test substance was incubated in a single mid loamy sand soil over a period of 28 days (carbon transformation) and 28 days (nitrogen transformation) at concentrations of 0.74 mg /kg soil dry weight and 3.68 mg/kg soil dry weight. Deviations from the control in nitrate levels, nitrate formation rates and rates of short-term respiration in soil treated with up to and including 3.68 mg/kg soil dry weight were low and all <25% indicating no significant effect occurred in carbon or nitrogen transformation. The derived EC10 and EC50 values were > 3.68 mg/kg soil dry weight.
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