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EC number: 601-485-7 | CAS number: 117570-93-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27-nov-2008 to 29-nov-2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study has been performed according to OECD and/or EC guidelines and according to GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-[2-(carboxymethyl)phenoxy]-3,4-dimethylbenzoic acid
- EC Number:
- 601-485-7
- Cas Number:
- 117570-93-1
- Molecular formula:
- C17 H16 O5
- IUPAC Name:
- 2-[2-(carboxymethyl)phenoxy]-3,4-dimethylbenzoic acid
- Details on test material:
- - Name of test material (as cited in study report): ASA404 C7
- Substance type: Off white powder
- Physical state: Solid
- Analytical purity: 99.9%
- Lot/batch No.: 072301
- Expiration date of the lot/batch: 18 March 2009
- Stability under test conditions: Stable
- Storage condition of test material: At room temperature in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all test concentrations and control
- Sampling method: Volume, 2 ml from the approximate centre of the test vessels at t=0 and t=48 h
- Sample storage conditions before analysis: Samples were stored in a freezer until analysis
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of test solutions started with a loading rate of 100 mg/l applying a 10-minute treatment period with ultrasonic waves followed by 74 hours of magnetic stirring to accelerate the dissolving of the test substance in the test medium. Obtained solutions contained undissolved material dispersed through and floating on the surface. The dispersion was consequently filtered through a 0.45 µm membrane filter (Whatman, RC55) to remove the non-dissolved material. The lower test concentrations were prepared by subsequent dilutions of the flitrate in test medium. The final test solutions were all clear and colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: In-house laboratory culture
- Age at study initiation (mean and range, SD): <24 hours
- Method of breeding: Start of each batch, With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium in an all-glass culture vessel.
- Feeding during test: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/l expressed as CaCO3
- Test temperature:
- 19.6 - 20.8 °C
- pH:
- At t=0: 6.3 - 7.9
At t=48: 7.1 - 8.0 - Dissolved oxygen:
- At t=0: 8.9 - 9.4
At t=48: 8.9 - Nominal and measured concentrations:
- Nominal concentrations: 0.1, 1, 10 and 100 mg/l
Measured concentrations:
At t=0: 96.5% of nominal
At t=48: 98% of initial - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml, all-glass
- Type (delete if not applicable): open - Material, size, headspace, fill volume: glass, ~80ml, normal headspace
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 for the highest test concentration, 2 in the lower test concentrations
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source preparation of dilution water: Adjusted ISO medium
- Culture medium different from test medium: yes for culturing M7 medium is used
OTHER TEST CONDITIONS
- Adjustment of pH: 7.7 ± 0.3
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS: EC50 daily
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no
- Mortality of control: no
- Other adverse effects control: not observed - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50/LC50:
The 24h-EC50 was 0.57 mg/l with a 95% confidence interval between 0.52 and 0.66 mg/l.
The 48h-EC50 was 0.35 mg/l with a 95% confidence interval between 0.31 and 0.40 mg/l.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- ASA404 C7 did not induce acute immobilisation of Daphnia magna at or below 10 mg/l after 48 hours of exposure (NOEC). A total of 40% immobile daphnia were observed at the highest test concentration of 100 mg/l.
Therefore, it is concluded that this study is valid and the 48h-EC50 exceeded 100 mg/l based on analytically confirmed nominal concentrations.
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