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EC number: 600-704-3 | CAS number: 10597-60-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19th March 2020 to 27th April 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-(2-hydroxyethyl)benzene-1,2-diol
- EC Number:
- 600-704-3
- Cas Number:
- 10597-60-1
- Molecular formula:
- C8H10O3
- IUPAC Name:
- 4-(2-hydroxyethyl)benzene-1,2-diol
1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: mixed treatment plant of urban (about 66%) and industrial (about 34%)liquid sewage situated at San Rocco Monza (MB), Italia.
- Storage conditions: the sampled muds were mixed and let settle and kept in aerobic conditions for 3 days.
- Preparation of inoculum for exposure: The mud samples, before their use, were analysed to check the ability to form colony units. Then it was centrifuged, washed and analysed to quantify the suspended solids concentration for the inoculum preparation.
- Concentration of sludge: The inoculum was prepared in order to have a concentration of suspended solid of about 4 mg/L. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 2.199 mg/L
- Based on:
- IC (inorganic carbon)
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- inorg. C analysis
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Stock Solution A: 8.5g/l of KH2PO4, 21.75g/l of K2HPO4, 33.40g/l of Na2HPO4.2H2O and 0.5g/l NH4Cl
Stock Solution B: 36.40g/l CaCl2 . 2H2O
Stock Solution C: 22.50g/L MgSO4 . 7H2O
Stock Solution D: 0.25g/l FeCl3 . 6H2O
To prepare 1L of mineral medium, 10ml of Stock Solution A was added to 800ml of distilled water, stirred and 1ml each of Stock Solutions B, C and D added. The mixture was then made up to 1L with distilled water.
- Test temperature: 20 ± 1 oC
- pH: 7.48
- Suspended solids concentration: 4 mg/L
Light: Incubation carried out in the dark
TEST SYSTEM
- Culturing apparatus: 1L flasks
- Number of culture flasks/concentration: 23 (blank, test sample, reference substance) 8 (test sample + reference substance, abiotic check)
SAMPLING
- Sampling frequency: 3 replica / once a week and 5 replica at day 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: Blank prepared containing water, culture medium and the inoculum of mud.
- Abiotic check: Test item with 50 mg/L HgCl2 in order to check for possible abiotic degradation..
- Toxicity control: Considering Sodium benzoate as reference substance using its molecular formula, a starting nominal concentration of 20.05 mg/L of organic carbon (TOC) has been used.
- Test sample + Reference substance: Test sample and reference substance in order to have a final concentration given by the sum of organic carbon (TOC) of the 2 substances.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 61
- Sampling time:
- 28 d
- Details on results:
- The mean amount of TIC present in the blank controls at the end of the test is < 3 mg C/L.
The solutions in the bottles containing the test item changed color from transparent to slightly orange during the test.
Abiotic degradation has occurred because the amount of TIC during the test in the abiotic bottles is greater than 10% so has been considered in the final percentage of biodegradation.
% of abiotic biodegradation at 28 days: 9%
Reference substance + test item confirms the absence of an inhibitory effect of the test sample on inoculum at the concentration at which it was applied in the test (4.62%).
BOD5 / COD results
- Results with reference substance:
- % BIODEGRADATION - 65%
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- On the basis of results obtained, interpreted in accordance to OECD 310:2014, the test item 'HYDROXYTYROSOL’ is considered readily biodegradable in aerobic conditions.
% of total aerobic biodegradation at 28 days: 61%
% of abiotic biodegradation at 28 days: 9% - Executive summary:
The analysis of inorganic carbon for the evaluation in an aqueous medium of the aerobic biodegradability of the test item, “HYDROXYTYROSOL”, has been performed following screening method described in OECD 310: 2014. For this purpose the amount of developed inorganic carbon has been measured and reported in comparison to a blank.
The test has been done using the test item with a fixed concentration of organic carbon (see Par. Assay Execution). Samples have been kept at the temperature of 20 ± 1 °C for the whole period of the test (28 days).
On the basis of results obtained, interpreted in accordance to OECD 310:2014, the test item 'HYDROXYTYROSOL’ is considered readily biodegradable in aerobic conditions.
% of total aerobic biodegradation at 28 days: 61%
% of abiotic biodegradation at 28 days: 9%
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