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EC number: 280-084-5 | CAS number: 82985-35-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 Jan - 22 Feb 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: Method for Testing the Degree of Accumulation of Chemical Substances in Fish Body, s tipulated in the Testing Methods for New Chemical Substances, Ministry of the Environment, Japan
- GLP compliance:
- yes
- Radiolabelling:
- no
- Details on sampling:
- - Sampling intervals/frequency for test organisms: analysis of test fish were performed 5 times during the exposure period. Test fish of control was analyzed before the experimental start and after the
experimental completion.
- Sampling intervals/frequency for test medium samples: test water of Level 1 and 2 were analyzed once before the first analysis of test fish and at the same time as analysis of test fish.
- Details on sampling and analysis of test organisms: 4 fish per treatment level were taken out at each sampling time and divided into 2 groups (2 fish per group) and analyzed individually. Test fish collection at final 3 successive analyses was carried out at intervals of more than 48 h, and the final analysis was performed at 28th d. Weight and body length of the fish was measured. Fish were chopped into pieces and refined. 4 – 5 g were mixed with 15 mL 5 mmol/L IPCC-MS7 in methanol and homogenize d for 1 min. Mixture was washed in 5 mL 5 mmol/L IPCC-MS7 in methanol and centrifuged at 7000 x g for 5 min. Supernatant was filtered and filled up to 50 mL with 5 mmol/L IPCC-MS7 in methanol. 1 mL was taken out and mixed with 5 mL of a 1/1 (v/v) mixture of 10 mmol/L IPCC-MS7 in methanol and ultra pure water. Filtrate was measured with LC-MS.
- Details on sampling and analysis of test media samples: 1 mL sample of test level 1 and 5 mL from test level 2 were collected. The sample of the test level 1 was filled up to 10 mL with water and 5 mL of this dilution was diluted 1:2 with 10 mmol/L IPCC-MS7 in methanol for LC-MS analysis. - Vehicle:
- no
- Details on preparation of test solutions, spiked fish food or sediment:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 104 mg/L stock solution was prepared by dissolving (stirring) the appropriate amount of the test item in ion exchanged water - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Common carp
- Source: CERI Kurume
- Age at study initiation (mean and range, SD): yearling fish
- Length at study initiation: 7.1 -7.7 cm
- Weight used for BCF calculation: 10 g
- Lipid content of fish: determined for control fish before and after the experiment with gravimetric analysis after chloroform-methanol extraction
- Feeding during test: yes, fish were starved for 24 h before sampling
- Food type: feed for fry of carp (proteins content > 43.0 %, lipid content > 3.0 %, Nippon Formula Feed Mfg. Co., Ltd.)
- Amount: 3 % of total body weight was fed
- Frequency: daily dose of food was split into two feedings
ACCLIMATION
- Acclimation period: 50 d
- External desinfection of fish: was carried out in an aqueous solution containing OTC for fisheries (oxytetracycline hydrochloride, Kyoritsu Seiyaku Corporation) and sodium chloride (The Salt Industry Center of Japan)
- Acclimation conditions (same as test or not): fish were acclimatized in a flow through system at 25 °C - Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
- Hardness:
- 15 mg/L (meas.)
- Test temperature:
- Level 1: 23.7-24.5 °C
Level 2: 24.0-25.0 °C
Control: 23.5-24.5 °C - pH:
- Level 1: 7.4 - 8.0
Level 2: 7.3 - 8.0
Control: 7.2 - 8.0 - Dissolved oxygen:
- Level 1: 7.3-8.1 mg/L
Level 2: 7.2-8.1 mg/L
Control: 7.2-8.1 mg/L - TOC:
- 0.8 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 70 L glass tank
- Aeration: yes
- Flow rate: 2 mL/min for stock solution, 400 mL/min for dilution water and control (579 L/d)
- No. of organisms per vessel: 24 at levels 1 and 2, 10 in the control
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: analyzed groundwater from the premises of CERI Kurume
- Suspended solid: <1 mg/L
- TOC: 0.8 mg/L
- Pesticides: <0.004 mg/L
- Chlorine: < 0.02 mg/L
- Intervals of water quality measurement: flow rate, water temperature were measured daily, dissolved oxygen was verified once per week and pH was recorded twice in the experimental period
- Intervals of observations: observation of test fish: twice a day
- Intervals of test medium replacement: flow - through system
OTHER TEST CONDITIONS
- Photoperiod: 14 h light /10 h dark
- Light intensity: artificial light of white fluorescent lamp - Nominal and measured concentrations:
- High exposure level (Level 1): 520 µg/L (nominal)
Low exposure level (Level 2): 52 µg/L (nominal) - Reference substance (positive control):
- no
- Type:
- BCF
- Value:
- < 0.44 dimensionless
- Basis:
- whole body w.w.
- Time of plateau:
- 28 d
- Remarks on result:
- other: Conc.in environment / dose:520 µg/L
- Type:
- BCF
- Value:
- < 4.2 dimensionless
- Basis:
- whole body w.w.
- Time of plateau:
- 28 d
- Remarks on result:
- other: Conc.in environment / dose:52 µg/L
- Details on results:
- - Behavioural abnormalities: none observed
- Other biological observations: lipid content of the test fish before test initiation: 3.23%, lipid content of the test fish after test completion: 3.05% (within the ± 25% range)
- Steady state: as the test item in all test fish at last three successive analyses were not higher than LOQ, BCFss was not calculated. However, because all BCFs were less than 100, it was evaluated that a steady-state was reached on the 28th day. - Validity criteria fulfilled:
- yes
- Conclusions:
- Bioconcentration factors were found to be BCF (28 d, 520 µg/L) < 0.44 and BCF (28 d, 52 µg/L) < 4.2 for Cyprinus carpio.
Reference
pH variation during the test period was 0,6 (Level 1) to 0.8 (Control), which was higher than the value stipulated in the test method (±0.5).
Concentrations of the test item were maintained > 88% of nominal concentrations and the variations were within ± 20% of the average measured concentrations.
Table 1: Measured concentrations of test item in test water in µg/L.
Level | 3 days | 10 days | 18 days | 21 days | 25 days | 28 days | Average (Standard deviation) |
1 | 455 | 485 | 481 | 518 | 520 | 528 | 498 (28.4) |
2 | 51.6 | 50.5 | 51.7 | 49.2 | 52.3 | 53.2 | 51.4 (1.41) |
Description of key information
BCF <0.44 whole body w.w. for the 520 µg/L test item concentration
BCF <4.2 whole body w.w. for 52 µg/L test item concentration
Key value for chemical safety assessment
Additional information
The substance hydrolyses rapidly to hydrolysis products, which have low log Kow values (<3) and thus have low potential for bioaccumulation. This was confirmed by a bioaccumulation study according to Method for Testing the Degree of Accumulation of Chemical Substances in Fish Body, stipulated in the Testing Methods for New Chemical Substances, Ministry of the Environment, Japan and GLP (2013). Cyprinus carpio as the test organism was exposed in a flow through system to two nominal exposure levels (52 and 520 µg/L) for 28 d. The test item was analytically measured via LC-MS. Hydrolysis of the test item was confirmed by LC-MS. Therefore the bioconcentration test was performed following bis(trihydroxysilylpropyl)amine as test item. The test item was recovered by > 88% in the test medium at the start and at the end of the experiment. BCF values of <0.44 and <4.2 whole body w.w. were determined for the 520 µg/L and the 52 µg/L concentrations respectively showing that no concern regarding bioaccumulation arises by the substance.
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