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EC number: 263-157-6 | CAS number: 61790-66-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- From March 20, 1991 to September 26, 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- No analytical dose verification.
- Justification for type of information:
- Refer to the section 13 for details on the category justification.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: CHODAT (86.81 SAG)
- Source (laboratory, culture collection): Own culture started from a smaple obtained from the 'Institut für Wassr-, Boden- und Lufthygiene, Berlin, Germany'
- Age of inoculum (at test initiation): 3 d - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22°C
- pH:
- 8.2 - 8.6 at test start; 8.4 - 9.1 at test end
- Nominal and measured concentrations:
- - Nominal: 0, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0 and 32.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Initial cells density: 20,000 cells/ml
- Control end cells density: 560,000 cells/ml
- No. of vessels per concentration (replicates): 5 - 8
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile conditions: yes
- Adjustment of pH: no
- Light intensity and quality: 8000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: photometric determination at 685 nm using a reference calibration curve. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 18.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 1.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 23.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the nominal 24 -72 h ErC50 was 18.6 mg/L. The nominal 72 h EbC50 was 23.4 mg/L and the NOEC was 2.0 mg/L.
- Executive summary:
A study was conducted to determine the toxicity of the test substance, C16-18 and C18-unsatd. DEA, to the green algae Scenedesmus subspicatus according to EU Method C.3, in compliance with GLP. Algae were exposed to the test substance (directly dissolved in test medium) for 72 h at 0, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0 and 32.0 mg/L under static conditions. Biomass and growth rate were determined. No analytical dose verification was conducted. During the incubation period, increased turbidity was noted at 8 mg/L and above. This was taken into account when calculating the toxicity data by subtracting the values for the parallel samples (same conditions, no algae) from the test sample (with algae).
Under the study conditions, the nominal 24 -72 h ErC50 was 18.6 mg/L. The nominal 72 h EbC50 was 23.4 mg/L and the NOEC was 2.0 mg/L (Scholz, 1991).
Reference
Table 1: Scenedesmus subspicatus cell density (10E+4 cell/ml) after exposure to the test substance
Concentration (mg/L) | 0 h | 24 h | 48 h | 72 h |
0 | 2 | 3 | 13 | 56 |
0.5 | 2 | 4 | 15 | 60 |
1.0 | 2 | 5 | 18 | 64 |
2.0 | 2 | 4 | 16 | 61 |
4.0 | 2 | 3 | 14 | 43 |
8.0 | 2 | 3 | 21 | 21 |
16.0 | 2 | 3 | 27 | 10 |
32.0 | 2 | 4 | 7 | 13 |
Description of key information
A study was conducted to determine the toxicity of the test substance, C16-18 and C18-unsatd. DEA, to the green algae Scenedesmus subspicatus according to EU Method C.3, in compliance with GLP. Algae were exposed to the test substance (directly dissolved in test medium) for 72 h at 0, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0 and 32.0 mg/L under static conditions. Biomass and growth rate were determined. No analytical dose verification was conducted. During the incubation period, increased turbidity was noted at 8 mg/L and above. This was taken into account when calculating the toxicity data by subtracting the values for the parallel samples (same conditions, no algae) from the test sample (with algae).Under the study conditions, the nominal 24 -72 h ErC50 was 18.6 mg/L. The nominal 72 h EbC50 was 23.4 mg/L and the NOEC was 2.0 mg/L (Scholz, 1991).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 18.6 mg/L
- EC10 or NOEC for freshwater algae:
- 2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.