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EC number: 246-791-8 | CAS number: 25291-17-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Only preliminary test.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- yes
- Remarks:
- Only a pre-test was performed for an exposure duration 30 min three replicates.
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture:
- Name and location of sewage treatment plant where inoculum was collected: Activated sludge obtained from an aeration reactor of a sewage treatment plant receiving predominantly domestic sewage (Kurume central sewage treatment center (Kurume-shi, Fukuoka, Japan)
- Method of cultivation: The activated sludge (5 L) was fed with the synthetic sewage (250 mL) and cultivated for 19.5 h at 20 ± 2 °C under aerobic condition in order to acclimate the test conditions. The activated sludge was then used. Acclimation to the test item was not performed. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Test temperature:
- 20 ± 2 °C
- Nominal and measured concentrations:
- nominal: control, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Approximately 300 mL glass vessel (test vessel); 102 mL glass incubator bottle
- Type (delete if not applicable): open
- Test solutions: The test sample (160 μL, i.e., 250 mg), the synthetic sewage (8 mL), the purified water (117 mL) and the activated sludge (125 mL) were added to each test vessel.
- Aeration: yes, flow rate 0.5 - 1.0 L/min.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 2
- Suspended solid concentration: 2330 mg/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified water
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): respiration rate after 30 min
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable, limit test - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Remarks on result:
- not measured/tested
- Remarks:
- Pre-test; no EC50 was calculated
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (30 min) 10 mg/L - Validity criteria fulfilled:
- not applicable
- Endpoint:
- activated sludge respiration inhibition testing
- Data waiving:
- study technically not feasible
- Justification for data waiving:
- other:
Referenceopen allclose all
Table 1: Inhibition of respiration
Test solution |
Concentration [mg/L] |
Oxygen consumption rate [mg O2/L/h] |
Percentage inhibition of respiration [%] |
Percentage residue of test item [%] |
Test substance 1 |
1000 |
44.0 |
8 |
4 |
Test substance 2 |
44.9 |
6 |
6 |
|
Test substance 3 |
40.3 |
16 |
0 |
|
Reference item |
4 |
37.3 |
22 |
- |
12 |
16.5 |
66 |
- |
|
36 |
7.85 |
84 |
- |
|
Blank control 1 |
- |
51.7 (beginning) |
- |
- |
Blank control 2 |
- |
43.9 (end) |
- |
- |
The preliminary test as simple test was valid because the results met validity criteria of the OECD 209 test, and the high recovery rates of the test item were obtained by the pretreatment of the test solution in the recovery test (96.4% and 94.8%).
Description of key information
Study technically not feasible.
Key value for chemical safety assessment
Additional information
An experimental study according to OECD 209 is technically not possible to be adequately performed. The substance has a vapour pressure of 4640 Pa at 25 °C and it is thus not possible to maintain exposure concentrations constant for the total exposure time of 3 h. An attempt was made to perform this study at a shorter duration of 30 min as recommended in ECHA Guidance R.7b (ECHA, 2017) for volatile substances (Daikin Industries, 2017). A test solution of nominal 1000 mg/L (three replicates) was aerated for 30 min according to OECD 209. This study was performed including chemical analysis by GC/FID to check if the substance is stable during exposure. Recovery rates were 96.4% and 94.8% (n=2) after preparation of the test solutions. After 30 min only 0-6% of the substance was found based on initial concentration. Thus, almost all of the substance evaporated after 30 min. Since it is not possible to perform the study with closed test vessels it was concluded that a main test is technically not possible to be performed since the substance concentration could not be maintained in the test solutions. In this pre-test no inhibition of respiration compared to the control was found after 30 min. The same process of evaporation would also happen at the aeration stage of common sewage treatment plants. Thus, it can be ensured that the test item is not likely to cause any effects on the degradation process of activated sludge microorganisms in sewage treatment plants.
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