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EC number: 246-788-1 | CAS number: 25279-09-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 January 2018 - 05 February 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- A sample of activated sludge was taken from the aeration tank of Sewage Treatment Plant ”Czajka”, Warsaw. The sludge was aerated for 6 days, at the test temperature of about 22 °C, until application. A sample was withdrawn just before use for the determination of the dry weight of the suspended solids. Before an application the sludge was washed in a mineral medium.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Prepare the following stock solutions, using analytical grade reagents.
(a) Monopotassium dihydrogen orthophosphate, KH2PO4 8.50 g
Dipotassium monohydrogen orthophosphate, K2HPO4 21.75 g
Disodium monohydrogen orthophosphate dihydrate Na2HPO4 · 2 H2O 33.40 g
Ammonium chloride, NH4Cl 0.50 g
Dissolve in water and make up to 1 litre The pH of the solution should be 7.4.
(b) Calcium chloride, anhydrous, CaCl2 27.50 g
Dissolve in water and make up to 1 litre
(c) Magnesium sulphate heptahydrate, MgSO4 · 7 H2O 22.50 g
Dissolve in water and make up to 1 litre.
(d) Iron(III) chloride hexahydrate, FeC13 · 6H2O 0.25 g
Dissolve in water and make up to 1 litre.
Mix 10 ml of solution (a) with 800 ml dilution water, add 1 ml of solutions (b) to (d) and make up to 11 with dilution water.
- Test temperature: 22 ºC
- pH: 7.40-8.46
- pH adjusted: no
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Thermo-cabinet WTW TS 606 CZ-G/3-VAR
- Number of culture flasks/concentration: Triplicate, containing test item (100 mg/L) and inoculum (30 mg/L SS).
- Measuring equipment:
Closed WTW OxiTop OC 110 respirometer for BOD determination;
Spectrophotometer Hach DR 3900 for TOC concentration measurements;
Electronic temperature recorder EBI 310 –T;
pH-meter – multifunctional microcomputer meter ELMETRON CX-505;
SAMPLING
The oxygen uptake from the readings taken at regular and frequent intervals was calculated. The data were read out every 112 min during the 28 day test (40 320 min that is 360 readings) and were recoded and stored in the measuring heads of the sample bottles. These collected data, using the controller, were infrared read out from the heads and stored in the controller. At the end of incubation, normally 28 days, the pH of the contents of the flasks was measured.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes (triplicate), only inoculum (30 mg/L SS)
- Toxicity control: Yes (triplicate), test item 100 mg/L, reference item (100 mg/L sodium acetate) and inoculum (30 mg/L SS) - Reference substance:
- acetic acid, sodium salt
- Remarks:
- 100 mg/L
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 76.5
- Sampling time:
- 28 d
- Details on results:
- At the 28th day of the test the measured aerobic biodegradation of the test item was 76.5%.
The pass level for ready biodegradability is 60% of ThOD. This value was attained after 14 days and was reached in a 10-day window.
Lag time was 4 day and degradation time was 15 days - Results with reference substance:
- The reference item reached 82.5% of biodegradation and the level for ready biodegradability by 4 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- (BOD differences between replicates < 20%, reference test item reached the pass level on day 4, in the toxicity test the biodegradation attained 42.4%, the oxigen update of inoculum blank was 48.2 mgO2/L in 28 days, the pH values were inside 6-8.5)
- Interpretation of results:
- readily biodegradable
- Conclusions:
- At the 28th day of the test the measured aerobic biodegradation of the test item was 76.5%. The pass level of ThOD of 60% was attained after 14 days and was reached in a 10-day window. Thus, the substance is determined to be readily biodegradable.
- Executive summary:
A ready biodegradation test was performed according to OECD Guideline 301F and EU Method C.4 -D (GLP study). A measured, stirred volume of a mineral solution containing 100 mg/L of test item was inoculated with microorganisms (activated sludge, 30 mg/L SS) and incubated under aerobic conditions (oxygen presence) in a closed respirometer flask at temperature of 22 ± 0.3 °C for 28 days. The blank tests were run in parallel with only inoculum but without test item. A reference item (sodium acetate) was run in parallel to check the operation of the procedures. The degradation was followed by the determination of oxygen uptake and measurements were taken at sufficiently frequent intervals to allow the identification of the beginning and end of biodegradation. To check the possible inhibitory effect of the test item the toxicity test was run in parallel. The solutions containing 100 mg/L, of both the test and a reference item, in the mineral medium, were inoculated. The consumption of oxygen was determined from the change in pressure in the apparatus. The carbon dioxide, evolved during test item degradation, was absorbed in a solution of potassium hydroxide. The amount of oxygen taken up by the test item (corrected for uptake by blank inoculum, run in parallel) was expressed as a percentage of calculated ThOD of the test item. At the 28th day of the test the measured aerobic biodegradation of the test item was 76.5%. The pass level of ThOD of 60% was attained after 14 days and was reached in a 10-day window. Thus, the substance is determined to be readily biodegradable. All the validity criteria were fullfilled. The reference item reached 82.5% of biodegradation and the level for ready biodegradability by 4 days. In the toxicity test the biodegradation was equal to 42.4% in 14 days, not being inhibitory.
The oxygen uptake of the inoculum blank was equal to 48.2 mg/L in 28 days.
The pH values of all flasks were inside the range 7.40-8.46.
Reference
The test item attained 76.5% of biodegradation.
The reference item reached 82.5% of biodegradation and the level for ready biodegradability by 4 days.
In the toxicity test the biodegradation was equal to 42.4% in 14 days, not being inhibitory.
The oxygen uptake of the inoculum blank was equal to 48.2 mg/L in 28 days.
The pH values of all flasks were inside the range 7.40-8.46.
|
time, days |
|||||||||||||
1 |
3 |
5 |
7 |
9 |
12 |
14 |
16 |
18 |
21 |
23 |
25 |
28 |
||
Test item O2 uptake, mg/L |
a1 |
7.4 |
23.9 |
65.5 |
105.4 |
135.1 |
172.0 |
189.8 |
203.1 |
212.8 |
223.5 |
231.0 |
235.9 |
244.1 |
a2 |
6.4 |
18.9 |
60.4 |
94.2 |
129.2 |
163.0 |
181.5 |
196.3 |
207.3 |
224.3 |
232.9 |
239.3 |
248.8 |
|
a3 |
11.5 |
21.3 |
66.2 |
99.5 |
135.0 |
172.2 |
189.0 |
203.1 |
211.7 |
226.1 |
232.9 |
239.4 |
248.2 |
|
am. avg |
8.4 |
21.3 |
64.0 |
99.7 |
133.1 |
169.1 |
186.8 |
200.8 |
210.6 |
224.6 |
232.3 |
238.2 |
247.0 |
|
Blank test O2 uptake mg/L |
b1 |
6.3 |
14.6 |
17.1 |
23.5 |
26.6 |
33.1 |
37.1 |
41.5 |
43.2 |
47.3 |
50.5 |
54.6 |
60.2 |
b2 |
7.4 |
11.2 |
15.5 |
17.9 |
23.2 |
29.3 |
29.9 |
31.7 |
34.9 |
38.0 |
37.9 |
42.3 |
44.2 |
|
b3 |
6.7 |
11.0 |
13.5 |
15.5 |
21.0 |
25.6 |
28.3 |
29.2 |
32.0 |
34.4 |
34.5 |
37.9 |
40.1 |
|
bm. avg |
6.8 |
12.2 |
15.4 |
19.0 |
23.6 |
29.3 |
31.7 |
34.1 |
36.7 |
39.9 |
41.0 |
44.9 |
48.2 |
|
Reference item O2 uptake. mg/L |
w1 |
24.2 |
51.6 |
62.9 |
73.9 |
82.1 |
87.5 |
92.6 |
95.8 |
98.5 |
102.5 |
105.2 |
107.6 |
111.7 |
w2 |
25.4 |
54.1 |
66.6 |
77.9 |
83.3 |
90.3 |
96.5 |
98.9 |
100.9 |
104.0 |
107.9 |
110.7 |
114.6 |
|
w3 |
25.4 |
55.8 |
67.9 |
77.9 |
83.2 |
90.2 |
93.7 |
97.2 |
99.0 |
102.8 |
104.7 |
107.0 |
111.2 |
|
wm. avg |
25.0 |
53.8 |
65.8 |
76.6 |
82.8 |
89.3 |
94.2 |
97.3 |
99.5 |
103.1 |
106.0 |
108.5 |
112.5 |
|
Toxicity control O2 uptake. mg/L |
tox1 |
26.1 |
57.3 |
78.1 |
102.1 |
125.7 |
151.4 |
165.2 |
174.4 |
184.7 |
196.4 |
202.2 |
208.3 |
216.8 |
tox2 |
22.0 |
53.8 |
73.5 |
97.1 |
121.1 |
154.4 |
173.8 |
192.3 |
208.3 |
231.9 |
244.5 |
256.0 |
270.0 |
|
tox3 |
22.7 |
57.1 |
79.9 |
103.4 |
128.2 |
164.0 |
185.8 |
204.2 |
218.8 |
240.0 |
251.4 |
266.2 |
286.2 |
|
toxm.avg |
23.6 |
56.1 |
77.1 |
100.9 |
125.0 |
156.6 |
175.0 |
190.3 |
204.0 |
222.8 |
232.7 |
243.5 |
257.7 |
|
Corrected test item O2 uptake, mg/L |
(a1-bm) |
0.6 |
11.6 |
50.1 |
86.5 |
111.6 |
142.7 |
158.0 |
169.0 |
176.1 |
183.7 |
190.0 |
191.0 |
195.9 |
(a2-bm) |
-0.4 |
6.6 |
45.0 |
75.2 |
105.6 |
133.7 |
149.8 |
162.2 |
170.6 |
184.4 |
191.9 |
194.4 |
200.7 |
|
(a3-bm) |
4.7 |
9.0 |
50.8 |
80.5 |
111.4 |
142.9 |
157.3 |
169.0 |
175.0 |
186.2 |
191.9 |
194.5 |
200.0 |
|
Reference item % degradation ThOD = 0.78 mgO2/mg C = 100 mg/L |
R1(w1) |
22.3 |
50.5 |
61.0 |
70.4 |
75.0 |
74.5 |
78.0 |
79.1 |
79.2 |
80.3 |
82.4 |
80.4 |
81.5 |
R2(w2) |
23.8 |
53.7 |
65.7 |
75.5 |
76.6 |
78.1 |
83.0 |
83.0 |
82.2 |
82.2 |
85.8 |
84.3 |
85.1 |
|
R3(w3) |
23.9 |
55.8 |
67.4 |
75.5 |
76.4 |
78.1 |
79.4 |
80.8 |
79.9 |
80.7 |
81.8 |
79.6 |
80.8 |
|
Rwavg |
23.3 |
53.3 |
64.7 |
73.8 |
76.0 |
76.9 |
80.1 |
81.0 |
80.5 |
81.1 |
83.3 |
81.4 |
82.5 |
|
Test item% degradation ThOD = 2.60 mgO2/mg C = 100 mg/L |
R1(a1) |
0.2 |
4.5 |
19.3 |
33.3 |
42.9 |
54.9 |
60.8 |
65.0 |
67.7 |
70.6 |
73.1 |
73.4 |
75.4 |
R2(a2) |
0.0 |
2.6 |
17.3 |
28.9 |
40.6 |
51.4 |
57.6 |
62.4 |
65.6 |
70.9 |
73.8 |
74.8 |
77.2 |
|
R3(a3) |
1.8 |
3.5 |
19.5 |
31.0 |
42.8 |
54.9 |
60.5 |
65.0 |
67.3 |
71.6 |
73.8 |
74.8 |
76.9 |
|
Raavg |
0.7 |
3.5 |
18.7 |
31.1 |
42.1 |
53.7 |
59.6 |
64.1 |
66.9 |
71.1 |
73.6 |
74.3 |
76.5 |
|
Toxicity test % degradation
|
R1(tox1) |
5.7 |
13.3 |
18.6 |
24.6 |
30.2 |
36.1 |
39.5 |
41.5 |
43.8 |
46.3 |
47.7 |
48.3 |
49.9 |
R2(tox2) |
4.5 |
12.3 |
17.2 |
23.1 |
28.9 |
37.0 |
42.0 |
46.8 |
50.8 |
56.8 |
60.2 |
62.4 |
65.6 |
|
R3(tox3) |
4.7 |
13.3 |
19.1 |
25.0 |
31.0 |
39.8 |
45.6 |
50.3 |
53.9 |
59.2 |
62.3 |
65.5 |
70.4 |
|
Rtoxavg |
5.0 |
13.0 |
18.3 |
24.2 |
30.0 |
37.7 |
42.4 |
46.2 |
49.5 |
54.1 |
56.7 |
58.7 |
62.0 |
Validity criteria:
- The difference of extremes of replicate values of the BOD of the test item at the plateau and at the end of the test is less than the limit of 20% or slightly exceeds this limit.
- The reference item has reached the pass level (60%) on day 4 (the limit is by day 14).
- In a toxicity test, containing both the test item and a reference item, on the 14th test day the biodegradation (based on ThOD) attained 42.4%.
- The oxygen uptake of the inoculum blank is 48.2 mg O2/l in 28 days (should not be greater than 60 mg/L).
- The pH values of all flasks were inside the range 6-8.5 and the oxygen consumption by the test item was greater than 60%.
Description of key information
Key study: OECD Guideline 301F and EU Method C.4 -D. GLP study. At the 28th day of the test the measured aerobic biodegradation of the test item was 76.5%. The pass level of ThOD of 60% was attained after 14 days and was reached in a 10-day window. Thus, the substance is determined to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
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