Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 245-423-3 | CAS number: 23089-26-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-09-05 to 2017-10-05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - CAS no.: 23089-26-1
- Batch no.: K80953370
- Purity: 97% (a/a) (GC) - Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples of each test medium concentration were taken directly after preparation and after 48 hours. One replicate was used for analysis.
- Sample storage conditions before analysis: Stored in a freezer at =>20 °C. At the day of analysis, the frozen samples were thrawed at RT. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A test solution with 100 mg/L was freshly prepared. For that purpose, the test item was weighed in a calibrated flask and vehicle (reconstituted water) was added. The preparation was stirred with a magnetic stirrer for 24 hours. Than the formulation was given into a separating funnel for 1 hour. The middle fraction in the separating funnel was used for the preparation of the different test item concentrations.
- Controls: Negative control, positive control with the reference item potassium dichromate
- Name of vehicle: Reconstituted water (as cited in the study)
- Evidence of undissolved material: No - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna Straus
- Source: Originally obtained from IBACON GmbH (Roßdorf, Germany); clone is bred in the laboratories of Merck KGaA
- Age of parental stock: Less than 24 hours old
- Feeding during test: No
CULTURE CONDITIONS: similar to test conditions
- Container: 100 mL glass vessels containing approximately 60 mL reconstituted water (ELENDT M4 medium)
- Water temperature: 20 +- 2 °C
- Light-cycle: 16 h light : 8 h darkness
- Feeding: Only during culturing period; ad libitium with unicellar green algae Pseudokirchneriella subcapitata three times per week
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS: Parental daphnids are used for the reproduction until they are about 6 weeks old. Thereafter, they are replaced by neonates. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- None
- Hardness:
- 236 mg CaCO3/L
- Test temperature:
- 20.6 to 20.9 °C
- pH:
- 7.86
- Dissolved oxygen:
- 8.64 mg/L
- Salinity:
- Not applicable
- Conductivity:
- 711 µS/cm
- Nominal and measured concentrations:
- - Nominal: 0.0 (control), 5.0, 9.0, 16.2, 29.2 and 52.5 mg/L
- Measured:
fresh medium:old medium, after 48 hours: - Details on test conditions:
- TEST SYSTEM
- Test vessel: Test tubes
- Type: Open
- Material, size, headspace, fill volume: Glass, 20 mL nominal volume, fill volume 20 mL
- Aeration: Test perfomed in air conditioned room, no air bubbling
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light : 8 h darkness
- Light intensity: 601 Lux and 598 Lux prior to ans at the end of the exposure period, respectively.
- Temperature: 20.6 to 20.9 °C
EFFECT PARAMETERS MEASURED: Mobility after 24 and 48 hours
RANGE-FINDING STUDY
- Test concentrations: = 0 (control), 1, 5, 10, 25, 50, 100 mg/L
- Results used to determine the conditions for the definitive study: Yes- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence interval: 2.9 - 4.2 mg/L
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.5 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence interval: 2.9 - 4.2 mg/L
- Details on results:
- In the control, not daphnids were immobilized. The dissolved oxygen concentration at the end of the exposure period was => 3 mg/L. Thus, the validity criteria were fulfilled.
- Results with reference substance (positive control):
- EC50 (48h): 0.74 mg/L (95% confidece interval: 0.66 - 0.82 mg/L)
- Reported statistics and error estimates:
- Estimation of ECx values according to Lichfield and Wilcoxon.
Nominal test item concentration [mg/L] Analytical results 0h 48h [mg/L] Recovery from nom. [%] [mg/L] Recovery from nom. [%] Recovery from 0h conc. [%] 0.0 <LOD - <LOD - - 5.0 0.844 16.9 0.763 15.3 90.4 9.0 1.584 17.6 1.472 16.4 92.9 16.2 3.05 18.8 2.684 16.6 88 29.2 5.231 17.9 5.028 17.2 96.1 52.5 9.501 18.1 8.818 16.8 92.8 - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study, the test item showed a 48-h EC50 of 2.2 mg/L.
- Executive summary:
To determine the effect of the test item on the mobility to Daphnia magna, an acute Immobilisation Test according to OECD Guideline 202 and EU Method C.2 was carried out in compliance with GLP principles. Juvenile daphnids (less than 24 hours old) were exposed to different test item concentrations of nominal 0.0 (blank control, only with ELENDT M4 Medium), 5.0, 9.0, 16.2, 29.2 and 52.5 mg/L in an open static system. 20 daphnids, devided into four replicates, each five animals were used per test item group and the control group. The daphnids were observed for immobilization after 24 and 48 hours of exposure. Samples of the test and control medium were taken directly after preparation and at the end of exposure (48 h) and stored at =< -20 °C until further analysis of the test item concentration via HPLC with UV detection. The measured concentrations were between 15.3% and 18.8% of the nominal concentrations. As the recoveries of the test item in the aqueous solutions were >80% of the initial concentrations during the study, the 24-h and 48-h EC50 values were calculated with the initial measured concentrations. In result, the test item showed a 48-h EC50 of 2.2 mg/L. All validity criteria of the guidelines were fulfilled.
Reference
Description of key information
The 48-h EC50 of the test item was determined to be 2.2 mg/L (based on initial measured concentrations).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.2 mg/L
Additional information
To determine the effect of the test item on the mobility to Daphnia magna, an acute Immobilisation Test according to OECD Guideline 202 and EU Method C.2 was carried out in compliance with GLP principles. Juvenile daphnids (less than 24 hours old) were exposed to different test item concentrations of nominal 0.0 (blank control, only with ELENDT M4 Medium), 5.0, 9.0, 16.2, 29.2 and 52.5 mg/L in an open static system. 20 daphnids, devided into four replicates, each five animals were used per test item group and the control group. The daphnids were observed for immobilization after 24 and 48 hours of exposure. Samples of the test and control medium were taken directly after preparation and at the end of exposure (48 h) and stored at =< -20 °C until further analysis of the test item concentration via HPLC with UV detection. The measured concentrations were between 15.3% and 18.8% of the nominal concentrations. As the recoveries of the test item in the aqueous solutions were >80% of the initial concentrations during the study, the 24-h and 48-h EC50 values were calculated with the initial measured concentrations. In result, the test item showed a 48-h EC50 of 2.2 mg/L. All validity criteria of the guidelines were fulfilled (reference 6.1.3 -1).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.