N-(1,4-dimethylpentyl)-N'-phenylbenzene-1,4-diamine

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles Lack of analytical monitoring data. Effect values should not be considered for risk evaluation.

Qualifier:

according to guideline

Guideline:

other: EPA-660/3-75-009

Deviations:

no

Principles of method if other than guideline:

EPA Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians. Environmental Protection Agency, Ecological Research Series
EPA-660/3-75-009, April, 1975. 61 p.
American Public Health Association. 1975. Standard Methods for the Examination of Water and Wastewater. 14th ed. Washington, DC. 1193 p.

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

Nanograde Acetone was used to prepare the test solutions and as the solvent control.

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

The bluegill sunfish used in the test were obtained from Osage Catfisheries in Osage Beach, Missouri.
All test fish were held in culture tanks on a 16 hour daylight photoperiod and observed for at least fourteen days prior to testing.
The bluegill sunfish used for this experiment had a mean weight of 0.14 (± 0.05) g and a mean standard length of 19 (± 1.9) mm.
The static fish bioassay was conducted in five gallon glass vessels containing 15 liters of ABC laboratory well water.
10 fish per vessel.

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

CaCO3 of 255 ppm

Test temperature:

21 °C

pH:

7.8 - 8.1

Dissolved oxygen:

6.9 - 8.7 (78-99 % saturation)

Nominal and measured concentrations:

nominal concentrations: 0.10, 0.18, 0.32, 0.56 and 1.0 mg/
All concentrations were observed once every 24 hours for mortality and abnormal effects.

Details on test conditions:

The test vessels were kept in a water bath at 22 °C (± 1.0). The test fish were acclimated to the dilution water and test temperature and held without food for 48 hours prior to testing.
A 96-hour range-finding test was conducted to determine the concentration range for the definitive study. The preliminary test concentrations were set at 0.1, 1.0, 10 and 100 mg/L. Based on the results of preliminary testing, five concentrations of the test compound, ranging in a logarithmic series from 0.10 to 1.0 mg/L, with ten fish per concentration were selected for definitive bioassay. Also included was a dilution water control and a solvent control chamber.

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

A 96-hour range-finding test was conducted to determine the concen tration range for the definitive study. The preliminary test concen trations were set at 0.1, 1.0, 10 and 100 mg/L. Based on the results of preliminary testing, five concentrations of the test compound, ranging in a logarithmic series from 0.10 to 1.0 mg/L, with ten fish per con centration were selected for definitive bioassay. Also included was a dilution water control and a solvent control chamber.

The bluegill sunfish were challenged with a reference compound, Antimycin A, to verify that the fish were in good condition. The 96 hour LC50 for bluegill sunfish exposed to Antimycin A was 7E-10 mg/L and was within the 95 % confidence intervals reported.

Statistical analysis of the concentration vs. effect data was obtained by employing a computerized program developed by Stephan et al. This program calculated the LC50 statistic and its 95 % confidence li mits using the binomial, the moving average, and the probit tests.

Description of key information

The acute toxicity of 7PPD to the freshwater fish, Lepomis macrochirus was investigated in a static test according to EPA-600/3-75-009. After 96 hours of exposure an LC50 of 0.3 mg/L (nominal) was determined. In addition to the Key Study, two additional acute toxicity sutdies used as Supporting Studies, were conducted with 7ppd exposed to rainbow trout (Oncorhynchus mykiss, formerly known as Salmo gairdneri). The resulting 96 -hr LC50 values were 0.4 and 0.42 mg/L (nominal).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Remarks:

(96 h)

Effect concentration:

0.3 mg/L