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EC number: 205-055-6 | CAS number: 132-27-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: Method described by Harries et al. (2000): "Development of a reproductive performance Test for endocrine-disrupting chemicals using pair-breeding Fathead minnows. Environmental Science and Technology 34, 3003-3011.
- Principles of method if other than guideline:
- This publication is a discussion document for a future OECD guideline.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 1, 5, 50, 500 µg/L
- Sampling time: day -1, 0, 1, 2, 8, 15, 16, 22 and 23 - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 300 mg/L stock solution was prepared by dissolving 1.8 g test substances in 6 L of dechlorinated water. The concentrate was stirred overnight to provide a clear and colourless solution. A series of stock solutions was prepared by addition of the required volume of stock concentrate to 10 L glass jars. - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: fathead minnow
- Source: brood stock held at Brixham Environmental Laboratory
- Length at study initiation:
- 62.8 -64.5 (male)
- 48.0 - 51.3 (female)
HOLDING CONDITIONS
- Holding period: 6 month
- Holding conditions: same as test, except that water was only filtered through a 10 µm filter before use
- Holding temperature: 25 ± 1 °C
- Type and amount of food: high protein pelleted fish food, supplemented with frozen brine shrimp
- Feeding frequency: daily
- Mortality: none within seven days prior test start
ACCLIMATION
- Acclimation period: 11 days
- Acclimation conditions: same as test - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 40.0 - 49.7 mg/L as CaCO3
- Test temperature:
- 24.7 - 25.5 °C
- pH:
- 7.3 - 7.8
- Dissolved oxygen:
- 6.4 - 8.8 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations: control, 1, 5, 50, 500 µg a.i./L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material, nominal volume, fill volume: glass vessels, 12 L, 9.5 L
- Type of flow-through: peristaltic diluter
- No. of organisms per vessel: pair of fish (1 male and 1 female)
- No. of vessels per concentration (replicates): 8 (6 adult and 2 progeny tanks)
- No. of vessels per control (replicates): 8 (6 adult and 2 progeny tanks)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water resevoir with an average retention time of 24 h. Passed through activated carbon, coarsly filtered to remove particulate material, dechlorinated with sodium thiosulphate and salts added, as required, to maintain minimum hardness levels. The treated water was held in a secondary resevoir with a capacity of 36 m³ and an average retention time of 8 h. The water was passed through an ultra violet steriliser to a second set of filters (25 and 10 µm mesh size) and then to a third storage tank with a capacity of 13.5 m³.
- Total organic carbon: 21.8 mg/L
- Alkalinity: 28.7 mg/L as CaCO3
- Ca/mg ratio: 12/3.2 mg/L
- Conductivity: 244 µS/cm
- Intervals of water quality measurement: Dissolved oxygen, pH and temperature measurements once per week in all test vessels; temperature measurements twice weekly
OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h dark, with a 10 min. dawn/dusk transition period
- Light intensity: 320-460 lux
EFFECT PARAMETERS MEASURED: fecundity (number of spawnings, total number of eggs spawned, number of eggs per spawning (egg batch size) and percent hatchability of eggs determination of plasma vittelogenin, (VTG), gonadosomatic index (GSI), histopathology - Reference substance (positive control):
- yes
- Remarks:
- 17α-ethynylestradiol
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.036 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.293 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to analogue justification provided in IUCLID section 13
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.036 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.293 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
Referenceopen allclose all
Biological parameter |
Effect concentration (µg a.i./L) |
|||
Nominal |
Mean measured |
|||
NOEC |
LOEC |
NOEC |
LOEC |
|
F0 Number of spawnings |
50 |
500 |
36 |
293 |
F0 Egg production |
50 |
500 |
36 |
293 |
F0 Egg batch size |
50 |
500 |
36 |
293 |
F0 GSI (male) |
500 |
>500 |
293 |
>293 |
F0 GSI (female) |
500 |
>500 |
293 |
>293 |
F0 Vitellogenin (male) |
500 |
>500 |
293 |
>293 |
F0 Vitellogenin (male) |
500 |
>500 |
293 |
>293 |
F1 Egg hatch |
50 |
500 |
36 |
293 |
Description of key information
NOEC (21 d): 0.036 mg a.i./L (measured, arithm. mean); read across
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.036 mg/L
Additional information
Data on the chronic toxicity of sodium 2-biphenylate (CAS 132-27-4) to fish are not available. The assessment was therefore based on a study conducted with the analogue substance 2-phenylphenol (CAS 90-43-7) as part of a read across approach, which is in accordance with Regulation (EC) No 1907/2006, Annex XI, 1.5. Further justification is given within the overall summary chapter 6.1 and within the analogue justification in IUCLID section 13.
The chronic toxicity of 2-phenylphenol to fathead minnow (Pimephales promelas) was tested in a reproductive performance test based on the method described by Harries et al. (2000) with GLP compliance. The test was set up as a flow-through system. The fish were exposed for 21 days to nominal test substance concentrations of 1, 5, 50, 500 µg a.i./L. The test substance concentrations were analytically verified at day -1, 0, 1, 2, 8, 15, 16, 22 and 23 and ranged from 59-81% of the nominal. The fecundity of test fish was assessed daily. Viability of resultant embryos was assessed in animals held in the same treatment regime to which the adults were exposed. A suite of histological and biological endpoints, that potentially are directly reflective of effects associated with endocrine disrupting chemicals, was also evaluated.
Based on arithmetic mean meas. concentration the overall NOEC (21 d) was determined as 0.036 mg a.i./L and the overall LOEC (21 d) as 0.293 mg a.i./L (measured, arithm. mean).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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