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Diss Factsheets
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EC number: 201-602-8 | CAS number: 85-40-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant study to recognised international guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,2,3,6-tetrahydrophthalimide
- EC Number:
- 201-602-8
- EC Name:
- 1,2,3,6-tetrahydrophthalimide
- Cas Number:
- 85-40-5
- Molecular formula:
- C8H9NO2
- IUPAC Name:
- 2,3,3a,4,7,7a-hexahydro-1H-isoindole-1,3-dione
- Details on test material:
- - Name of test material (as cited in study report): THPI D4
- Physical state: Solid
- Analytical purity: 97.91%
- Lot/batch No.: STI4208324
- Expiration date of the lot/batch: 2009-11-21
- Stability under test conditions: Stable
- Storage condition of test material: Room temperature
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal sewage treatnment plant at d-31137 Hildesheim, receiving principally domestic sewage
- Pretreatment: Received sludge washed with sterile water, settled, then re-suspended in mineral salts medium. Maintained in aerobic condition by aeration for 4 hours, After this the sludge was homogenised in a blender, the supernatant decanted and mainatined under aerobic conditions for 5 days
- Initial cell/biomass concentration: 10E+7 - 10E+8 CFU/L - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 40 mg/L
- Based on:
- test mat.
- Initial conc.:
- 70 mg/L
- Based on:
- ThOD/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium as described in OECD test guideline 310F
- Additional substrate: None
- Solubilising agent (type and concentration if used): None
- Test temperature: 20.5 - 22.0 Deg C
- pH: 7.31 - 7.60
- pH adjusted: No
- Aeration of dilution water: No
- Suspended solids concentration: To give 10E+7 - 10E+8 CFU/L in test vessels
- Continuous darkness: Yes
TEST SYSTEM
- Culturing apparatus: Brown glass bottles
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Continuous stirring
- Measuring equipment: Automated system (OxiTop) measuring pressure differences following adsorption of carbon dioxide
- Test performed in closed vessels due to significant volatility of test substance: No
- Details of trap for CO2 and volatile organics if used: Soad lime trap
SAMPLING
- Sampling frequency: For oxygen consumption - every 112 minutes for 28 days
- Sampling method: Automated
- Sterility check if applicable: Not applicable
- Sample storage before analysis: Not applicable
:
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes - 2 vessels
- Abiotic sterile control: No
- Toxicity control: Yes - 1 vessel
- Other: Functional (positive) control - 1 vessel
STATISTICAL METHODS: None used
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 30
- St. dev.:
- 7.1
- Sampling time:
- 28 d
BOD5 / COD results
BOD5 / COD
- Parameter:
- BOD5
- Value:
- 70 mg O2/g test mat.
- Results with reference substance:
- >60% biodegradation achieved after 2 days,
Any other information on results incl. tables
Biodegradation (%) | ||||
Day |
Positive control |
Test substance | Toxicity control | |
1 | 2 | |||
0 | 0 | 0 | 0 | 0 |
1 | 53 | 0 | 0 | 28 |
2 | 67 | 2 | 0 | 37 |
3 | 7 | 2 | 0 | 44 |
4 | 82 | 4 | 4 | 48 |
5 | 85 | 5 | 3 | 49 |
6 | 85 | 1 | 1 | 50 |
7 | 88 | 2 | 2 | 51 |
8 | 90 | 2 | 2 | 51 |
9 | 89 | 3 | 0 | 52 |
10 | 91 | 3 | 0 | 53 |
11 | 92 | 2 | 0 | 53 |
12 | 95 | 3 | 1 | 54 |
13 | 97 | 3 | 3 | 54 |
14 | 99 | 4 | 4 | 55 |
15 | 100 | 4 | 4 | 55 |
16 | 100 | 3 | 9 | 56 |
17 | 100 | 4 | 10 | 56 |
18 | 100 | 6 | 10 | 57 |
19 | 100 | 9 | 9 | 58 |
20 | 100 | 11 | 9 | 59 |
21 | 100 | 15 | 11 | 59 |
22 | 100 | 19 | 11 | 59 |
23 | 100 | 26 | 14 | 58 |
24 | 100 | 35 | 13 | 59 |
25 | 97 | 36 | 16 | 58 |
26 | 100 | 38 | 28 | 57 |
27 | 97 | 32 | 22 | 48 |
28 | 98 | 35 | 25 | 48 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- Substance is not readily biodegradable
- Executive summary:
Substance is not readily biodegradable
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